| 产品编号 | bs-1620R |
| 英文名称 | CDX2 Rabbit pAb |
| 中文名称 | 尾型同源盒转录因子2抗体 |
| 别 名 | cau-dal-related homeobox transcription factor 2; Caudal type homeo box 2; Caudal type homeobox protein 2; caudal type homeobox transcription factor 2; CDX 2; CDX 3; CDX3; Homeobox protein CDX-2; Homeobox protein CDX2; CDX2_HUMAN. |
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Specific References (2) | bs-1620R has been referenced in 2 publications.
[IF=6.208] Shuai Yu. et al. KDM4C Contributes to Trophoblast-like Stem Cell Conversion from Porcine-Induced Pluripotent Stem Cells (piPSCs) via Regulating CDX2. INT J MOL SCI. 2022 Jan;23(14):7586 IF, WB ; Pig.
[IF=2.489] Dandan Chai. et al. CDX2 and Reg IV expression and correlation in gastric cancer. Bmc Gastroenterol. 2021 Dec;21(1):1-12 IHC ; Human.
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| 研究领域 | 肿瘤 发育生物学 染色质和核信号 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDX2: 151-250/313 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012] Function: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylation of Ser-60 mediates the transactivation capacity. Similarity: Belongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q99626 Gene ID: 1045 Database links: Entrez Gene: 1045 Human Entrez Gene: 12591 Mouse Omim: 600297 Human SwissProt: Q99626 Human SwissProt: P43241 Mouse Unigene: 174249 Human Unigene: 20358 Mouse Unigene: 64495 Rat 核转录因子抗体 CDX2表达主要用于消化系统肿瘤及癌前病变的研究。用于与肿瘤的增值、分化、大小、临床分期及分化程度等关系方面的研究。CDX2是一种肠特异的转录因子，CDX2能够调节肠上皮细胞的增殖和分化，定位于肠上皮的绒毛和隐窝表面。据报道95%以上的浸润性结/直肠癌中均可检出CDX2阳性表达，也可在胃癌和卵巢黏液性腺癌中表达。 |
| 产品图片 |
Sample:
Lane 1: Lovo (Human) Cell Lysate at 30 ug
Lane 2: FHC (Human) Cell Lysate at 30 ug
Primary: Anti-CDX2 (bs-1620R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDX2) Polyclonal Antibody, Unconjugated (bs-1620R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDX2) Polyclonal Antibody, Unconjugated (bs-1620R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
