产品编号 | bs-0965R |
英文名称 | FLAG Tag |
中文名称 | FLAG Tag标签(N端)抗体 |
别 名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag; DDDDK tag (N-T). |
Specific References (4) | bs-0965R has been referenced in 4 publications.
[IF=4.096] Liyan Wang. et al. Tracing Key Molecular Regulators of Lipid Biosynthesis in Tuber Development of Cyperus esculentus Using Transcriptomics and Lipidomics Profiling. Genes-Basel. 2021 Oct;12(10):1492 WB ; Cyperus esculentus.
[IF=3.688] Jiangbo Qu. et al. Functional differentiation of two lhx8 paralogs and possible regulatory role of lhx8a in Japanese flounder (Paralichthys olivaceus). Gene. 2022 Apr;817:146201 WB,IP ; Human.
[IF=3.046] Ghoorun RA et al. Prognostic Significance of FKBP14 in Gastric Cancer. Onco Targets Ther. 2019 Dec 30;12:11567-11577. WB ;
[IF=2.741] Zhao, Mengmeng. et al. TRIM4-mediated ubiquitination of NSP2 restricts porcine reproductive and respiratory syndrome virus proliferation. BMC VET RES. 2022 Dec;18(1):1-7 IP ; Human.
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产品类型 | 标签抗体 |
研究领域 | 细胞生物 免疫学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Recombinant protein |
产品应用 | WB=1:1000-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | MAP of Flag Tag peptide C-terminus |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This is a useful tool for the localisation and characterisation of DYKDDDDK tagged proteins. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用抗DYKDDDDK标签8肽抗体检测DYKDDDDK标签N端8肽融合蛋白 1) DYKDDDDK标签8肽与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3) DYKDDDDK标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物) 4) DYKDDDDK8肽标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能,不会影响蛋白分泌 5) DYKDDDDK8肽标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。 |
产品图片 | |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |