| 产品编号 | bs-1560R |
| 英文名称 | Reelin |
| 中文名称 | 络丝蛋白抗体 |
| 别 名 | Reelin; Reeler; RELN; RL; LIS2; PRO1598; RELN_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Chicken,Dog,Pig,Horse,GuineaPig) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 400-450;300;180-kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RELN: 3345-3458/3458 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in “reeler” mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1 in a signaling pathway that controls cell positioning in the developing brain and is also thought to be a direct effector of the neurotrophin BDNF. Function: This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. Subcellular Location: Secreted Tissue Specificity: High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level). Post-translational modifications: N-glycosylated; more than 90% of the glycans are sialylated. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P78509 Gene ID: 5649 Database links: Entrez Gene: 5649 Human Entrez Gene: 19699 Mouse Omim: 600514 Human SwissProt: P78509 Human SwissProt: Q60841 Mouse Unigene: 655654 Human Unigene: 425236 Mouse Unigene: 98353 Rat Reelin蛋白作用目前还在进一步研究中，但有学者认为它与β-Amyloid 的神经缠结有相对应的作用。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Reelin) Polyclonal Antibody, Unconjugated (bs-1560R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
