| 产品编号 | bs-1611R |
| 英文名称 | Cathepsin K |
| 中文名称 | 组织蛋白酶K抗体 |
| 别 名 | Cathepsin K; Procathepsin K; Cathepsin K precursor; Cathepsin O; Cathepsin O1; Cathepsin O2; Cathepsin X; CathepsinK; CTS02; CTSK; CTSK protein; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; Pycnodysostosis; cath-K; CK. |
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Specific References (11) | bs-1611R has been referenced in 11 publications.
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| 研究领域 | 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse (predicted: GuineaPig,Horse,Cow,Pig,Dog,Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cathepsin K: 112-210/329 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008]. Function: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Subunit: Belongs to the peptidase C1 family. Subcellular Location: Lysosome. Tissue Specificity: Tissue specificityPredominantly expressed in osteclasts (bones). DISEASE: Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Similarity: Belongs to the peptidase C1 family. SWISS: P43235 Gene ID: 1513 Database links: Entrez Gene: 1513 Human Entrez Gene: 13038 Mouse Omim: 601105 Human SwissProt: P43235 Human SwissProt: P55097 Mouse Unigene: 632466 Human Unigene: 272085 Mouse Unigene: 5598 Rat 合成与降解（Synthesis and Degradation） 组织蛋白酶K(cathepsinK)是半胱氨酸蛋白酶家族的成员,它和组织蛋白酶S、L和B有很高的同源性。 在心血管方面：组织蛋白酶K参与心血管的很多病理生理过程，在动脉硬化、血管重塑与再生。也是动脉瘤生长、破裂、主动脉辩狭窄进程中主要的胶原酶之一。 在骨与关节方面：组织蛋白酶K主要位于破骨细胞，是涉及骨质再吸收的主要酶，与骨质疏松与脱钙密切相关。 |
| 产品图片 |
Sample:
Lane 1: Mouse Raw264.7 cell lysates Lane 2: Mouse 4T1 cell lysates Primary: Anti-Cathepsin K (bs-1611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 46 kDa 
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated(bs-1611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
