| 产品编号 | bs-1753R |
| 英文名称 | ECE2 |
| 中文名称 | 内皮素转化酶2抗体 |
| 别 名 | ECE2; MGC78487; ECE 2; Endothelin converting enzyme 2; Hypothetical protein MGC2408; KIAA0604; MGC17664; ECE2_HUMAN. |
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Specific References (1) | bs-1753R has been referenced in 1 publications.
[IF=5.23] Esposito, Giuseppe, et al. "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration." Scientific reports 6 (2016). IHC-F ; Rat.
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| 研究领域 | 免疫学 神经生物学 转录调节因子 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 80-100kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ECE2: 601-700/883 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Endothelin-converting enzyme 2 (ECE2) converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Expressed at high levels in central nervous system. Expressed in adrenal glands, ovary and uterus, and at low levels in heart. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic granule membrane; Single-pass type II membrane protein (By similarity). Tissue Specificity: Expressed in brain. Strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). Similarity: In the N-terminal section; belongs to the methyltransferase superfamily. In the C-terminal section; belongs to the peptidase M13 family. SWISS: O60344 Gene ID: 9718 Database links: Entrez Gene: 9718 Human Omim: 610145 Human SwissProt: O60344 Human Unigene: 146161 Human |
| 产品图片 |
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-ECE2 (bs-1753R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80-100 kD Observed band size: 80 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
