| 产品编号 | bs-1787R |
| 英文名称 | GATA6 |
| 中文名称 | GATA结合蛋白6抗体 |
| 别 名 | Gata binding factor 6; Gata binding protein 6; GATA-binding factor 6; Gata6; GATA6_HUMAN; Transcription factor Gata 6; Transcription factor GATA-6. |
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Specific References (1) | bs-1787R has been referenced in 1 publications.
[IF=2.198] Liu, Wen. et al. Transcriptome sequencing of LMP2A-transfected gastric cancer cells identifies potential biomarkers in EBV-associated gastric cancer. VIRUS GENES. 2022 Jul;:1-12 WB ; Human.
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| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 发育生物学 染色质和核信号 干细胞 转录调节因子 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GATA6: 451-550/595 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]. Function: Transcriptional activator that regulates SEMA3C and PLXNA2. Thought to be important for regulating terminal differentiation and/or proliferation. Subunit: Interacts with LMCD1 (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed in heart, gut and gut-derived tissues. DISEASE: Defects in GATA6 are a cause of conotruncal heart malformations (CTHM) [MIM:217095]. A group of congenital heart defects involving the outflow tracts. Examples include truncus arteriosus communis, double-outlet right ventricle and transposition of great arteries. Truncus arteriosus communis is characterized by a single outflow tract instead of a separate aorta and pulmonary artery. In transposition of the great arteries, the aorta arises from the right ventricle and the pulmonary artery from the left ventricle. In double outlet of the right ventricle, both the pulmonary artery and aorta arise from the right ventricle. Note=GATA6 mutations have been found in patients with non-syndromic persistent truncus arteriosus (PubMed:19666519). Defects in GATA6 are the cause of atrial septal defect type 9 (ASD9) [MIM:614475]. A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Some patients manifest tricuspid valve disease, pulmonary valve disease, and pulmonary artery hypertension. Defects in GATA6 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. A congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. In this condition, blood from both ventricles (oxygen-rich and oxygen-poor) is pumped into the body often causing cyanosis. Defects in GATA6 are the cause of atrioventricular septal defect type 5 (AVSD5) [MIM:614474]. A congenital heart malformation characterized by a common atrioventricular junction coexisting with deficient atrioventricular septation. The complete form involves underdevelopment of the lower part of the atrial septum and the upper part of the ventricular septum; the valve itself is also shared. A less severe form, known as ostium primum atrial septal defect, is characterized by separate atrioventricular valvar orifices despite a common junction. Defects in GATA6 are a cause of pancreatic agenesis and congenital heart defects (PACHD) [MIM:600001]. An autosomal dominant disease characterized by pancreatic severe hypoplasia or agenesis, diabetes mellitus, and congenital heart abonormalities including ventricular septal defect, patent ductus arteriosus, pulmonary artery stenosis, truncus arteriosus and tetralogy of Fallot. Similarity: Contains 2 GATA-type zinc fingers. SWISS: Q92908 Gene ID: 2627 Database links: Entrez Gene: 2627 Human Entrez Gene: 14465 Mouse Omim: 601656 Human SwissProt: Q92908 Human SwissProt: Q61169 Mouse Unigene: 514746 Human Unigene: 329287 Mouse Unigene: 8701 Rat GATA6又称转录调节因子GATA6，可能抑制Wnt路径，方式是通过直接调控Wnt路径中另一种名为Fzd2的蛋白的表达。而Wnt路径是干细胞生物学中一个主要的路径。GATA6负调控Wnt路径并且其已被证明在胚胎干细胞复制和分化中也起重要作用。 |
| 产品图片 |
Sample:
Heart (Mouse) Lysate at 40 ug Primary: Anti-GATA6 (bs- 1787R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD 
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GATA6) Polyclonal Antibody, Unconjugated (bs-1787R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
