| 产品编号 | bs-1933R |
| 英文名称 | WNT9A |
| 中文名称 | 信号通路Wnt9a抗体 |
| 别 名 | wingless-type MMTV integration site 9A; MGC124412; Protein Wnt 9b precursor; Wingless type MMTV integration site 9B; Wingless type MMTV integration site family member 15; Wingless type MMTV integration site family member 9B; Wnt 14b; Wnt-15; Wnt14b; Wnt15; Wnt9b wingless type MMTV integration site family member 9B; WNT14; WNT9A_HUMAN. |
| 研究领域 | 肿瘤 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT9A: 21-120/365 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expressed in adult brain, lung, skeletal muscle, heart, and 17-day embryo. Similarity: Belongs to the Wnt family. SWISS: O14904 Gene ID: 7483 Database links: Entrez Gene: 7483 Human Entrez Gene: 216795 Mouse Omim: 602863 Human SwissProt: O14904 Human SwissProt: Q8R5M2 Mouse Unigene: 149504 Human Unigene: 218794 Mouse Unigene: 40411 Rat |
| 产品图片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WNT9A Polyclonal Antibody, Unconjugated(bs-1933R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
