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Rabbit Anti-MMP9 antibody (bs-2099R)
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说明书: 50ul  100ul  200ul
50ul/780.00元
100ul/1280.00元
200ul/1980.00元
大包装/询价

产品编号 bs-2099R
英文名称 MMP9
中文名称 基质金属蛋白酶9抗体
别    名 Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV. Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; Matrix metalloproteinase-9; 92 kDa type IV collagenase; Gelatinase B; 67 Da matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9.  
规格价格 50ul/780元 购买    100ul/1280元 购买    200ul/1980元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  细胞生物  神经生物学  信号转导  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
细胞定位 细胞核 细胞膜 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human MMP9 C-terminus
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 产 商 BIOSS
PubMed PubMed
产品介绍 background:
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). TIMPs have been shown to block tumor cell invasion suggesting that they act as metastasis suppressor genes.

Function:
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Subunit:
Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1.

Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).

Tissue Specificity:
Produced by normal alveolar macrophages and granulocytes.

Post-translational modifications:
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.

DISEASE:
Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

Similarity:
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.

SWISS:
P14780

Gene ID:
4318

Database links:

Entrez Gene: 4318 Human

Entrez Gene: 403885 Dog

Entrez Gene: 17395 Mouse

Entrez Gene: 81687 Rat

Omim: 120361 Human

SwissProt: O18733 Dog

SwissProt: P14780 Human

SwissProt: P41245 Mouse

SwissProt: P50282 Rat

Unigene: 297413 Human

Unigene: 4406 Mouse

Unigene: 10209 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

合成与降解(Synthesis and Degradation)
MMP9亦称IV型胶原酶或明胶酶B,其主要功能为降解IV型胶原。因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。
目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与转移浸润的研究。细胞外基质在维持正常组织结构与功能以及细胞生长和分化过程中起重要作用。细胞外基质动态平衡的失调与肿瘤细胞侵袭、转移和复发密切相关,基质金属蛋白酶(MMP9)是细胞外基质的降解酶,可降解Ⅳ、Ⅴ、Ⅸ、Ⅺ型胶原,在肿瘤的浸润、转移过程中起重要作用,近年为肿瘤研究的热点。
产品图片
Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-9 Polyclonal Antibody, Unconjugated(bs-2099R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
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