| 产品编号 | bs-1963R |
| 英文名称 | KLK1 |
| 中文名称 | 激肽释放酶1抗体 |
| 别 名 | Kallikrein 1; Kallikrein-1; hK1; Klk6; KLKR; Glandular kallikrein 1; hK 1; Kallikrein serine protease 1; Kidney/pancreas/salivary gland kallikrein; Klk 6; Tissue kallikrein; Klk1b3; Klk1c1; Ngfg; rGK-1; preprokallikrein; KLK1_HUMAN. |
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Specific References (1) | bs-1963R has been referenced in 1 publications.
[IF=0] Morrison MD et al. Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case-Control Study. Head Neck Pathol. 2019 Sep;13(3):286-297. IHC-P ; Human.
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| 研究领域 | 肿瘤 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 26kDa |
| 细胞定位 | 细胞核 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLK1: 101-200/262 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008] Function: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Tissue Specificity: Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. Post-translational modifications: The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. SWISS: P06870 Gene ID: 3816 Database links: Entrez Gene: 3816 Human GenBank: NP_002248.1 Human Omim: 147910 Human SwissProt: P06870 Human Unigene: 123107 Human Unigene: 11331 Rat 激肽释放酶KLK是激肽系统的主要限速酶，它是一组存在于多数组织和体液中的丝氨酸蛋白酶，是一种肽链内切酶。KLK又称血管舒缓素，包括15个家族成员。在不同的组织中广泛表达,具有蛋白水解酶的活性。它特异性的在碳末端切割底物肽，可裂解激肽原释放具有活性的激肽，由激肽发挥对心血管系统及肾脏功能的调节作用。 组织KLK是一个大的基因家族，主要分布在肺、肾、血管、脑、肾上腺组织，为一种中等大小的糖蛋白。 |
| 产品图片 |
Sample:
Lane 1: Human K562 cell lysates Lane 2: Human SW480 cell lysates Primary: Anti-KLK1 (bs-1963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 30 kD 
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
