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Rabbit Anti-LAMP1 antibody (bs-1970R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1970R
英文名称 LAMP1
中文名称 溶酶体相关膜蛋白1抗体
别    名 CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1; OTTHUMP00000040663.  
Specific References  (8)     |     bs-1970R has been referenced in 8 publications.
[IF=9.11] Sun, Ming-Xia, et al. "Porcine reproductive and respiratory syndrome virus induces autophagy to promote virus replication." Autophagy 8.10 (2012): 1434-1447.  IF(ICC) ;  Monkey.  
[IF=8.4] Dixit, Saurabh, et al. "Caveolin-mediated endocytosis of the Chlamydia M278 outer membrane peptide encapsulated in poly (lactic acid)-Poly (ethylene glycol) nanoparticles by mouse primary dendritic cells enhances specific immune effectors mediated by MHC class II and CD4+ T cells." Biomaterials (2017).  IF(ICC) ;  Mouse.  
[IF=6.854] Goreczny et al. Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1. (2018) Oncogene. 37:1699-1713  IHC ;  Human.  
[IF=6.43] Pan, Pan, et al. "Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer." Frontiers in Immunology 8 (2017): 997.  IHC-P ;  Mouse.  
[IF=6.43] Pan et al. Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer. (2017) Front.Immunol. 8:997  IHC-P ;  Mouse.  
[IF=5.55] Bechor, Sapir, et al. "Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2017).  WB ;  Mouse.  
[IF=4.37] Guo, Hong, et al. "Niemann Pick type C2 deficiency impairs autophagy-lysosomal activity, mitochondrial function, and TLR signaling in adipocytes."Journal of Lipid Research (2016): jlr-M066522.  WB ;  Mouse.  
[IF=1.69] Jeong, Keun-Yeong, et al. "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." Journal of Radiation Research (2014): rru077.  WB ;  Mouse.  
研究领域 肿瘤  免疫学  神经生物学  信号转导  干细胞  新陈代谢  细胞膜蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LAMP-1:301-417/417 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Function:
Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Post-translational modifications:
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.

Similarity:
Belongs to the LAMP family.

SWISS:
P11279

Gene ID:
3916

Database links:

Entrez Gene: 3916 Human

Entrez Gene: 16783 Mouse

Entrez Gene: 25328 Rat

Omim: 153330 Human

SwissProt: P11279 Human

SwissProt: P11438 Mouse

SwissProt: P14562 Rat

Unigene: 494419 Human

Unigene: 16716 Mouse

Unigene: 475822 Mouse

Unigene: 40177 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD107a可很好的反映NK细胞活性,其表达水平的上调与NK分泌细胞因子的增多及NK对靶细胞的杀伤作用的增强高度相关,采用流式检测,CD107a还可鉴定出一群无细胞因子分泌的活性NK细胞。
产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (bs-1970R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (bs-1970R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-cy3) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Blank control (blue line): U937 (blue)(fixed with 2% paraformaldehyde for 10 min at room temperature).
Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (bs-1970R); Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<; Dilution: 1μg /test.
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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