| 产品编号 | bs-2405R |
| 英文名称 | AKR1B1 |
| 中文名称 | 醛糖还原酶抗体 |
| 别 名 | MGC134297; ADR; aldehyde reductase 1; Aldehyde reductase; Aldose reductase; aldr 1; ALDR1; low Km aldose reductase. |
| 研究领域 | 心血管 免疫学 激酶和磷酸酶 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AKR1B1: 151-250/316 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009] Function: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. Similarity: Belongs to the aldo/keto reductase family. SWISS: P15121 Gene ID: 231 Database links: Entrez Gene: 231 Human Entrez Gene: 11677 Mouse Entrez Gene: 100009122 Rabbit Omim: 103880 Human SwissProt: P15121 Human SwissProt: P45376 Mouse SwissProt: P15122 Rabbit Unigene: 521212 Human Unigene: 451 Mouse Unigene: 485031 Mouse Unigene: 107801 Rat 醛糖还原酶是多元醇通路的关键限速酶,当血糖在正常生理水平时，它并不激活，它对葡萄糖的亲和力较低。在高血糖状况下（如糖尿病），磷酸己糖激酶饱和， 醛糖还原酶激活，促使体内的葡萄糖转化为山梨醇。然而，山梨醇脱氢酶（sorbitol dehydrogenase，SDH）的活力并未相应地成比例增加，山梨醇转化为果糖的效率没有提高,山梨醇本身由于极性强不易通过细胞膜，在细胞内会形成蓄积，使细胞膜的通透性发生改变，并使细胞中Na+-K+-ATP酶活性下降，造成肌醇丧失，导致细胞代谢与功能的损害。 因眼睛和神经细胞等组织内醛糖还原酶的含量较高，糖尿病病人体内高血糖的环境使这一通路很容易被打开，造成对相关组织的病理损害，如糖尿病、白内障、神经病变、肾脏病变、视网膜病变、动脉粥样硬化等糖尿病并发症DCC的发生。 |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug Primary: Anti-AKR1B1 (bs-2405R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 34 kD 
Sample:
Testis (Mouse) Lysate at 40 ug Primary: Anti-AKR1B1 (bs-2405R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 34 kD 
Sample:
Lane 1: Mouse Adrenal gland tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Adrenal gland tissue lysates Lane 4: Rat Testis tissue lysates Lane 5: Human HepG2 cell lysates Lane 6: Human A549 cell lysates Lane 7: Human U87MG cell lysates Primary: Anti-AKR1B1 (bs-2405R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
