产品中心-北京博奥森生物技术有限公司

产品编号	bs-2442R
英文名称	MyoD1/Myf3 Rabbit pAb
中文名称	肌原调节蛋白抗体
别名	Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3.
	Specific References (17) \| bs-2442R has been referenced in 17 publications. [IF=6.064] Sanghun Park. et al. Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat. BIOMOLECULES. 2022 Jun;12(6):838 IF ; Bovine. PubMed:35740963 [IF=5.924] Jing Zhang. et al. LncRNA SMARCD3-OT1 Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation via Enhancing SMARCD3X4 Expression. INT J MOL SCI. 2022 Jan;23(9):4510 WB ; Chicken. PubMed:35562902 [IF=5.895] Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):10121–10133 WB ; Pig, Mouse. PubMed:35960196 [IF=5.209] Xianglun Zhang. et al. Protective effect of resveratrol against hydrogen peroxide-induced oxidative stress in bovine skeletal muscle cells. Meat Sci. 2022 Mar;185:108724 IF ; Cattle. PubMed:34952489 [IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212 WB ; Mouse. PubMed:10.1016/j.biocel.2022.106212 [IF=4.873] Siqi Liu. et al. Effects of lycopene on skeletal muscle-fiber type and high-fat diet-induced oxidative stress. J Nutr Biochem. 2021 Jan;87:108523 WB ; Mouse. PubMed:33039582 [IF=4.534] Weng S et al. Improvement of muscular atrophy by AAV–SaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975. WB ; Mouse. PubMed:32398787 [IF=3.405] Dawson LE et al. Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines. Sarcoma. 2020 Mar 26;2020:8647981. FC ; human. PubMed:32300280 [IF=3.231] Pengfei Shi. et al. Analysis of Promoter Methylation of the Bovine FOXO1 Gene and Its Effect on Proliferation and Differentiation of Myoblasts. ANIMALS. 2023 Jan;13(2):319 WB ; Bovine. PubMed:36670858 [IF=2.884] Jindi Zhao. et al. Mutation c.−379 C>T in DGAT1 affects intramyocellular lipid content by altering MYOD1 binding affinity. ANIM GENET. 2023 Mar;: IP ; Pig. PubMed:36871966 [IF=2.788] Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9 WB ; Pig. PubMed:35570234 [IF=2.698] Koike, Tatiana E.. et al. Glutamine supplementation improves contractile function of regenerating soleus muscles from rats. J Muscle Res Cell M. 2022 Feb;:1-11 WB ; Rat. PubMed:35201551 [IF=2.39] Dai, Yang, et al. "microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1." Molecular and Cellular Biochemistry (2016): 1-10. WB ; Bovine. PubMed:26833195 [IF=1.977] Jie Li. et al. MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7. Biotechnol Lett. 2020 Sep;42(9):1633-1644 WB ; Mouse. PubMed:32382971 [IF=1.949] Kou YT et al. A transient protective effect of low-level laser irradiation against disuse-induced atrophy of rats. Lasers Med Sci. 2019 Apr 4. ICF ; Rat. PubMed:30949786 [IF=0] Shiratsuchi, Takaki, et al. "Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells." Journal of St. Marianna University 6.2 (2015): 225-233. IF(ICC) ; Human. PubMed:J.St.MariannaUniv.6,225–233,2015 [IF=0] Gong, Tao, et al. "The Control of Mesenchymal Stem Cell Differentiation Using Dynamically Tunable Surface Microgrooves." Advanced Healthcare Materials (2014). Rat. PubMed:24648133
研究领域	免疫学发育生物学转录调节因子
抗体来源	Rabbit
克隆类型	Polyclonal
克隆号
交叉反应	Mouse,Rat
产品应用	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	35 kDa
检测分子量
细胞定位	细胞核
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from mouse MyoD1: 51-150/318
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] Function: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function. Ubiquitinated on the N-terminus; which is required for proteasomal degradation Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P10085 Gene ID: 17927 Database links: Entrez Gene: 374048 Chicken Entrez Gene: 281938 Cow Entrez Gene: 4654 Human Entrez Gene: 17927 Mouse Entrez Gene: 407604 Pig Entrez Gene: 337868 Rat Entrez Gene: 443405 Sheep Omim: 159970 Human SwissProt: P16075 Chicken SwissProt: Q7YS82 Cow SwissProt: P15172 Human SwissProt: P10085 Mouse SwissProt: P49811 Pig SwissProt: Q02346 Rat SwissProt: P29331 Sheep Unigene: 181768 Human Unigene: 1526 Mouse Unigene: 9493 Rat
产品图片	25 ug total protein per lane of various lysates (see on figure) probed with MyoD1/Myf3 polyclonal antibody, unconjugated (bs-2442R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记