| 产品编号 | bs-2503R |
| 英文名称 | EEF1A1 Rabbit pAb |
| 中文名称 | 翻译延伸因子EF-1a抗体 |
| 别 名 | Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu(EF-Tu); Eukaryotic elongation factor 1 A-1(eEF1A-1); Leukocyte receptor cluster member 7; EF1A1_HUMAN; EEF1A; EF1A; LENG7; |
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Specific References (1) | bs-2503R has been referenced in 1 publications.
[IF=4.074] Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res
. 2020 Aug 7;19(8):3211-3219. WB ; Cow.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EEF1A1: 131-230/462 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subcellular Location: Cytoplasm. Tissue Specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle Post-translational modifications: ISGylated. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. SWISS: P68104 Gene ID: 1915 Database links: Entrez Gene: 1915 Human Entrez Gene: 13627 Mouse SwissProt: P68104 Human SwissProt: P10126 Mouse 肽链延长因子1A-1(peptide elongation factor 1A-1,eEF1A-1)是真核细胞蛋白翻译过程中肽链延长复合物的重要构成成分,具有调节蛋白合成及促发凋亡等多种作用. |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EF1a/eEF1A1 Polyclonal Antibody, Unconjugated(bs-2503R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
