[IF=6.684] Ma X. et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.. Front Cell Dev Biol. 2022 Feb;10:791986-791986  IHC ;  Human.  

PubMed:35237595

[IF=5.62] Lin, Xuzhu, et al. "Hindlimb Immobilization, but not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated with Muscle Atrophy in Rats." Journal of Bone and Mineral Research (2016).  WB ;  Rat.  

PubMed:27291707

[IF=3.412] Zhang Quan-Bing. et al. Effect of Electrical Stimulation on Disuse Muscular Atrophy Induced by Immobilization: Correlation with Upregulation of PERK Signal and Parkin-Mediated Mitophagy. AM J PHYS MED REHAB. 2023 Jan;:10.1097/PHM.0000000000002182  IHC ;  Rabbit.  

PubMed:36630294

Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAFbx/Fbx32 Polyclonal Antibody, Unconjugated(bs-2591R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Fbx32 antibody (bs-2591R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.