| 产品编号 | bs-2623R |
| 英文名称 | IL-22 Rabbit pAb |
| 中文名称 | 白介素-22抗体 |
| 别 名 | Interleukin-22; Il22; Iltif; ILTIFa; MGC129416; MGC129417; IL-10-related T-cell-derived-inducible factor; IL-TIF; ILTIF; IL-TIF alpha; Interleukin-22a; IL-22a; IL 21; IL D110; IL TIF; IL21; IL22; ILD110; interleukin 21; MGC79382; MGC79384; TIFa; TIFIL 23; TIFIL-23; TIFIL23; zcyto18; Cytokine Zcyto18. |
 |
Specific References (5) | bs-2623R has been referenced in 5 publications.
[IF=8.755] Jieying Chen. et al. Nestin+ Peyer's patch resident MSCs enhance healing of inflammatory bowel disease through IL-22-mediated intestinal epithelial repair. CELL PROLIFERAT. 2022 Nov;:e13363 WB ; Mouse.
[IF=3.061] Wanzhen Li. et al. Ac2-26 attenuates hepatic ischemia-reperfusion injury in mice via regulating IL-22/IL-22R1/STAT3 signaling. PEERJ. 2022 Sep;10:e14086 WB ; Mouse.
[IF=2.09] Huang, Yong-Hong, et al. "Th22 cell accumulation is associated with colorectal cancer development."World Journal of Gastroenterology IHC-P ;
[IF=1.42] Karaboga, İhsan, Selim Demirtas, and Turan Karaca. "Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats." Iranian Journal of Basic Medical Sciences 20.8 (2017): 870-879. WB ; Rat.
[IF=0] Zhang, Yan, and Mikhail G. Kolonin. "Cytokine signaling regulating adipose stromal cell trafficking." Adipocyte 5.4 (2016): 369-374. IHC-P ; Mouse.
|
| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-22: 35-179/179 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri. [provided by RefSeq]. Function: Cytokine that contributes to the inflammatory response in vivo. Subcellular Location: Secreted. Similarity: Belongs to the IL-10 family. SWISS: Q9GZX6 Gene ID: 50616 Database links: Entrez Gene: 50616 Human Entrez Gene: 50929 Mouse Omim: 605330 Human SwissProt: Q9GZX6 Human SwissProt: Q9JJY9 Mouse Unigene: 287369 Human Unigene: 103585 Mouse Unigene: 195879 Rat IL-22是白介素-10家族的成员,主要是由激活的T 淋巴细胞产生的一类重要的细胞因子,除了在Th17细胞中高表达之外，在NK和NKT等细胞中也有表达,在组织损伤修复以及牛皮癣、肝炎、肺炎、肠炎等慢性炎症中发挥重要作用.IL-22是继IL-6和IL-10之后又一个参与炎症反应和代谢调控的细胞因子，IL-22有可能成为干预脂肪肝发生发展的新方法。 |
| 产品图片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-IL22 (bs-2623R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD
Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-IL22 (bs-2623R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (bs-2623R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-IL22 antibody(bs-2623R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
