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Rabbit Anti-JNK1+JNK2+JNK3  antibody (bs-2592R)
~~~促销，代码KX240301~~~
~~~促销，代码KX240302~~~
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说明书： 50ul  100ul  200ul
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产品编号 bs-2592R
英文名称 JNK1+JNK2+JNK3
中文名称 氨基末端激酶1/2/3抗体
别    名 JNK1 + JNK2 + JNK3; JNK1/2/3; JNK1+2+3; JNK1 + JNK2 + JNK3; MAPK10; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; JNK; JNK1; JNK2; JNK2ALPHA; JNK2BETA; JNK3; MAPK8; MAPK9; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9; SAPK(beta); Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA.  
Specific References  (35)     |     bs-2592R has been referenced in 35 publications.
[IF=8.039] Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse.  
[IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453  WB ;  Human.  
[IF=6.656] Mingjuan Yang. et al. Rosmarinic acid potentiates and detoxifies tacrine in combination for Alzheimer's disease. PHYTOMEDICINE. 2022 Dec;:154600  WB ;  Mouse.  
[IF=5.923] Junfeng Ke. et al. CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway. Int J Mol Sci. 2021 Jan;22(22):12229  WB,IF ;  Human.  
[IF=5.455] Zhang, Rongrong. et al. Compound traditional Chinese medicine dermatitis ointment ameliorates inflammatory responses and dysregulation of itch-related molecules in atopic dermatitis. Chin Med-Uk. 2022 Dec;17(1):1-19  WB ;  Mouse.  
[IF=4.868] Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175.  WB ;  Rat.  
[IF=4.868] Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823.  WB ;  broiler.  
[IF=4.486] Jianye Yang. et al. Astragalus polysaccharide attenuates LPS-related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway. 2021 Jun 02  WB ;  Mouse.  
[IF=4.478] Dan-Ping Xie. et al. Peroxiredoxin 5 protects HepG2 cells from ethyl β-carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways. J CANCER. 2022 Sep 6;13(11):3258-3267  WB ;  Human.  
[IF=4.225] Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664  WB ;  Mouse.  
[IF=4.171] Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brain–liver axis in rats. 2021 May 18  WB ;  Rat.  
[IF=3.829] Wen, Yukang. et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages. VET RES. 2022 Dec;53(1):1-15  WB ;  Pig.  
[IF=3.829] Zhang Xiangjun. et al. DAD3 targets ACE2 to inhibit the MAPK and NF-κB signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells. VET RES. 2022 Dec;53(1):1-13  WB ;  Bovine.  
[IF=3.738] Lili Liu. et al. Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-κB Pathway. 2021 Jun 06  WB ;  Chicken.  
[IF=3.69] Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873  WB ;  Human.  
[IF=3.69] Pan Li et al. Curcumin metabolites contribute to the effect of curcumin on ameliorating insulin sensitivity in high-glucose-induced insulin-resistant HepG2 cells. J Ethnopharmacol. 2020 Sep 15;259:113015.  WB ;  Human.  
[IF=3.654] Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-κB Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108  IF,WB ;  Human.  
[IF=3.641] Wenbo Ge. et al. 17β-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21  WB ;  Sheep.  
[IF=3.322] Shu Zeng. et al. Inhibition of mGluR5 ameliorates lipid accumulation and inflammation in HepG2 cells. BIOCHEM BIOPH RES CO. 2023 Apr;653:1  WB ;  Human.  
[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.  
[IF=3.166] Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124.  WB ;  Piglet.  
[IF=3.118] Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6.  WB ;  Human.  
[IF=3.118] Jiang Y et al. Changes in inflammatory factors and protein expression in sulfur mustard (1LD 50)-induced acute pulmonary injury in rats.International Immunopharmacology, 2018 61, 338–345.  IHC-P ;  Rat.  
[IF=2.693] Xzaviar K.V. Solone. et al. MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. 2022 Feb 19  IF ;  Human.  
[IF=2.678] Gerson G Contreras-Chavez. et al. Changes in Appetite Regulation-Related Signaling Pathways in the Brain of Mice Supplemented with Non-nutritive Sweeteners. 2020 Oct 31  WB ;  Mouse.  
[IF=2.65] Yiming Bi. et al. β-Sitosterol Suppresses LPS-Induced Cytokine Production in Human Umbilical Vein Endothelial Cells via MAPKs and NF-κB Signaling Pathway. EVID-BASED COMPL ALT. 2023 Jan 03;2023:9241090  WB ;  Human.  
[IF=2.42] Li, Mingyong, et al. "c-Jun N-Terminal Kinase is Upregulated in Patients With Hypospadias." Urology 81.1 (2013): 178-183.  IHC-P ;  Human.  
[IF=2.394] Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.  IHC-P ;  Mouse.  
[IF=2.352] Wang X et al. Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway.(2018) Toxicon.18:30386-6  WB ;  piglet.  
[IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.  WB ;  Mouse.  
研究领域 细胞生物  细胞凋亡  糖尿病  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Pig,Cow)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 42-47kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human JNK1/2/3: 151-250/384 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 JNK1(MAPK8) is a member of the MAP kinase family. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.
JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
JNK3 is a neuron-specific form of c-Jun N-terminal kinases. Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis.
The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity.


SWISS:
Q61831

Gene ID:
5599

Database links:

Entrez Gene: 5599 Human

Entrez Gene: 26419 Mouse

Entrez Gene: 116554 Rat

Omim: 601158 Human JNK1

SwissProt: P45983 Human JNK1

Unigene: 138211 Human JNK1

Unigene: 21495 Mouse JNK1

Unigene: 4090 Rat JNK1

Entrez Gene: 5601 Human JNK2



产品图片
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Sample:
Hela(Human) CellLysate at 30 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Heart(Rat) Lysate at 40 ug
Heart(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Cerebellum(Mouse) Lysate at 40 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46'54 kD
Observed band size: 54 kD
Sample:
K562 (Human) Lysate at 30 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-47 kD
Observed band size:42-52 kD
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/54 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-JNK1/2/3 Polyclonal Antibody, Unconjugated(bs-2592R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (JNK1+JNK2+JNK3) polyclonal Antibody, Unconjugated (bs-2592R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-2592R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-2592R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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