产品编号 | bs-2639R |
英文名称 | PTGES2 |
中文名称 | 前列腺素E合成酶2抗体 |
别 名 | C9orf15; FLJ14038; Gamma interferon activated transcriptional element binding factor 1; GATE binding factor 1; GBF 1; GBF1; Membrane associated prostaglandin E synthase 2; MGC11289; Microsomal prostaglandin E synthase 2; mPGES 2; mPGES-2; PGES2; PGES2_HUMAN; Prostaglandin E synthase 2; Prostaglandin E synthase 2 truncated form; PTGES 2; PGES2_MOUSE. |
Specific References (10) | bs-2639R has been referenced in 10 publications.
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研究领域 | 肿瘤 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 43kDa |
细胞定位 | 细胞核 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse PTGES2: 281-384/384 <Cytoplasmic> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq]. Function: Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. Subunit: Homodimer. May interact with CEBPB. Subcellular Location: Golgi apparatus membrane; Single-pass membrane protein. Nucleus. Note=According to PubMed:12050152, some fraction may be nuclear. Prostaglandin E synthase 2 truncated form: Cytoplasm. Note=Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. Tissue Specificity: Widely expressed. Expressed in brain, heart, liver, colon and lung. Similarity: Belongs to the GST superfamily. Contains 1 glutaredoxin domain. Contains 1 GST C-terminal domain. SWISS: Q8BWM0 Gene ID: 96979 Database links: Entrez Gene: 80142 Human Entrez Gene: 96979 Mouse Entrez Gene: 100294563 Sheep Omim: 608152 Human SwissProt: Q9H7Z7 Human SwissProt: Q8BWM0 Mouse Unigene: 495219 Human Unigene: 28048 Mouse |
产品图片 |
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |