| 产品编号 | bs-2668R |
| 英文名称 | Bone sialoprotein |
| 中文名称 | 骨涎蛋白抗体 |
| 别 名 | BNSP; Bone sialoprotein II; BSP; BSPII; Cell binding sialoprotein; Integrin binding sialoprotein; SPII; SIAL_HUMAN; Bone sialoprotein 2; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein. |
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Specific References (6) | bs-2668R has been referenced in 6 publications.
[IF=6.344] Zheng, Han. et al. miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition. Int J Oral Sci. 2021 Dec;13(1):1-10 WB ; Human.
[IF=5.37] Yang, Zhenhua, et al. "Cessation of epithelial Bmp signaling switches the differentiation of crown epithelia to the root lineage in a β-catenin-dependent manner." Molecular and Cellular Biology (2013): MCB-00456. Mouse.
[IF=4.963] Shi R et al. Analysis of the characteristics and expression profiles of coding and noncoding RNAs of human dental pulp stem cells in hypoxic conditions.Stem Cell Res Ther. 2019 Mar 12;10(1):89. WB ; Human.
[IF=3.23] Potter AS, Potter SS (2015) Molecular Anatomy of Palate Development. PLoS ONE 10(7): e0132662. doi:10.1371 IHC-P ; Mouse.
[IF=3.078] Jilong Zou. et al. Resveratrol Benefits the Lineage Commitment of BMSCs into Osteoblasts via MiR‐320c by Targeting Runx2. 2021 Jan 22 WB ; Human.
[IF=2.677] Xing Yifeng. et al. Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy. J ORTHOP SURG RES. 2023 Dec;18(1):1-13 WB ; Rat.
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| 研究领域 | 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Bone sialoprotein II: 21-120/317 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008] Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. Subcellular Location: Secreted. Post-translational modifications: N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains. Sulfated on either Tyr-320 or Tyr-321 (By similarity). SWISS: P21815 Gene ID: 3381 Database links: Entrez Gene: 3381 Human Entrez Gene: 15891 Mouse Omim: 147563 Human SwissProt: P21815 Human SwissProt: Q61711 Mouse Unigene: 518726 Human Unigene: 4987 Mouse Unigene: 9721 Rat |
| 产品图片 |
Sample:
Lane 1: Bone (Mouse) Lysate at 40 ug Lane 2: Bone (Rat) Lysate at 40 ug Primary: Anti-Bone sialoprotein (bs-2668R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
