产品中心-北京博奥森生物技术有限公司

产品编号	bs-2075R
英文名称	Heme Oxygenase 1
中文名称	血红素氧合酶 1/热休克蛋白32抗体
别名	Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN.
	Specific References (34) \| bs-2075R has been referenced in 34 publications. [IF=7.338] Giraud S et al. The inhibition of eIF5A hypusination by GC7, a preconditioning protocol to prevent brain death‐induced renal injuries in a preclinical porcine kidney transplantation model. Am J Transplant. 2020 Dec;20(12):3326-3340. WB ; Pig. PubMed:32400964 [IF=7.129] Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546 WB ; Chicken. PubMed:36646010 [IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;: WB ; Rat. PubMed:10.1016/j.jare.2021.04.002 [IF=6.244] Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282 WB ; Mouse. PubMed:34307161 [IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine WB ; Human. PubMed:23434766 [IF=5.195] Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758 WB ; Rat. PubMed:36167232 [IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498. WB ; Human. PubMed:32695257 [IF=4.96] L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemia–reperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;180:114194 WB ; Rat. PubMed:32800851 [IF=4.64] Song, Wei, et al. "Astroglial Heme Oxygenase-1 and the Origin of Corpora Amylacea in Aging and Degenerating Neural Tissues." Experimental Neurology (2014). IHC-P ; Mouse. PubMed:24440642 [IF=4.571] Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297 WB ; Rat. PubMed:10.1016/j.tox.2022.153297 [IF=4.545] Yingran Liang. et al. Crocin ameliorates arsenic trioxide‑induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713 WB ; Rat. PubMed:32920515 [IF=4.527] Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239. WB ; Chicken. PubMed:31991393 [IF=4.49] Wang, Yun, et al. "TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity." Oxidative Medicine and Cellular Longevity 2017 (2017). WB ; Rat. PubMed:28303170 [IF=4.407] Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134 WB ; Mouse. PubMed:34247099 [IF=4.24] Wei et al. The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats. (2014) Atherosclerosis. 235:318-27 WB ; Rat. PubMed:24911635 [IF=4.105] Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1α proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963 WB ; Human. PubMed:33444648 [IF=3.79] Bai, Juan, et al. "Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats." Toxicology and Applied Pharmacology (2017). WB ; Rat. PubMed:28842207 [IF=3.73] Huanhuan Wang. et al. The Pigments in Eggshell with Different Color and the Pigment Regulatory Genes Expression in Corresponding Chicken’s Shell Gland. ANIMAL. 2023 Mar;:100776 WB,IHC ; Chicken. PubMed:37043933 [IF=3.71] Wei, Bo, et al. "Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats."Atherosclerosis (2013). WB ; Rat. PubMed:23958267 [IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996 IHC ; Rat. PubMed:33684450 [IF=3.117] Li J et al. Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Vet Res. 2019 Oct 21;50(1):83. WB ; Chicken. PubMed:31639045 [IF=3.056] Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946 WB ; Chicken. PubMed:32827115 [IF=3.04] Tawfik KM et al. Neuroprotective mechanisms of sildenafil and selenium in PTZ-kindling model: Implications in epilepsy.Eur J Pharmacol. 2018 Aug 15;833:131-144. IHC-P ; Mouse. PubMed:29807031 [IF=2.81] Maïga, Souleymane, et al. "Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model." PLOS ONE 12.7 (2017): e0181067. WB ; Pig. PubMed:28704481 [IF=1.858] Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-β/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09 WB ; Dog. PubMed:35678492 [IF=1.69] Xuan et al. Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation. (2016) Mol.Med.Rep. 14:2697-704 IHC-P ; Rat. PubMed:27484589 [IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27 WB ; Bovine. PubMed:33866621 [IF=1.581] Ni et al. Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling. (2015) Int.J.Clin.Exp.Pathol. 8:7752-61 WB ; Rat. PubMed:26339340 [IF=1.58] Zhang et al. Protective effect of heme oxygenase-1 on Wistar rats with heart failure through the inhibition of inflammation and amelioration of intestinal microcirculation. (2015) J.Geriatr.Cardiol. 12:353-65 WB ; Rat. PubMed:26346675 [IF=1.55] Wang, Yun, et al. "Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats." Molecular Medicine Reports 15.2 (2017): 673-680. WB ; Rat. PubMed:28035393
研究领域	肿瘤心血管免疫学神经生物学信号转导表观遗传学 Alzheimer's
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat,Pig (predicted: Chicken,Dog,Cow,Horse,Rabbit,Sheep,GuineaPig)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	32kDa
细胞定位	细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human HO-1: 101-200/288
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The hemeoxygenase-1 calls that the hemoglobin oxidizes to synthesize the enzyme again-1( hemeoxygenase-1, HO-1) is the catalyst enzyme that a kind of hemoglobin declines the solution, under the NADPH and the cell dye P-450 revivification enzymes and the member oxygen functions, the catalyst HO-1 hemoglobin declines the solution as the courage green vegetable, CO and irons, the former revivification has the very strong anti- to oxidize the ability after become the red vegetable of courage , the latter is a kind of important letter to make the member. Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Subcellular Location: Microsome. Endoplasmic reticulum. Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). DISEASE: Defects in HMOX1 are the cause of heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]. A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly. Similarity: Belongs to the heme oxygenase family. SWISS: P09601 Gene ID: 3162 Database links: Entrez Gene: 442987 Dog Entrez Gene: 3162 Human Entrez Gene: 15368 Mouse Entrez Gene: 24451 Rat Omim: 141250 Human SwissProt: Q5E9F2 Cow SwissProt: P09601 Human SwissProt: P14901 Mouse
产品图片	Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Lane 3: DU145 (Human) Cell Lysate at 30 ug Lane 4: H9C2 (Rat) Cell Lysate at 30 ug Lane 5: 293T (Human) Cell Lysate at 30 ug Primary: Anti-Heme Oxygenase 1 (bs-2075R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 35 kD Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Heme Oxygenase 1 Polyclonal Antibody, Unconjugated(bs-2075R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记