| 产品编号 | bs-2715R |
| 英文名称 | THBS1 |
| 中文名称 | 血小板反应蛋白抗体 |
| 别 名 | THBS 1; THBS-1; THBS; Thrombospondin 1; Thrombospondin1; Thrombospondin-1; Tsp 1; TSP-1; TSP; Tsp1. |
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Specific References (6) | bs-2715R has been referenced in 6 publications.
[IF=4.932] Qifeng Deng. et al. Cordycepin enhances anti-tumor immunity in colon cancer by inhibiting phagocytosis immune checkpoint CD47 expression. Int Immunopharmacol. 2022 Jun;107:108695 WB ; Mouse.
[IF=4.803] Duan F et al. THBS1 regulates trophoblast fusion through a CD36-dependent inhibition of cAMP, and its upregulation participates in preeclampsia. Genes & Diseases, (2020), S2352304220300726–. WB ; Human.
[IF=4.025] Li Zhuo-Hang. et al. The roles of ADAMDEC1 in trophoblast differentiation during normal pregnancy and preeclampsia. MOLECULAR HUMAN REPRODUCTION. 2022 May;: WB ; Human.
[IF=3.412] Xiuli Mei. et al. Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit. Front Vet Sci. 2022 Mar 10;9:838802 IHC ; Rabbit.
[IF=1.39] Zhang et al. Correlation between TSP-1, TGF-β and PPAR-γ expression levels and glioma microvascular density. (2014) Oncol.Let. 7:95-100 IHC-P ; Human.
[IF=1.39] Zhang et al. Correlation between TSP-1, TGF-β and PPAR-γ expression levels and glioma microvascular density. (2014) Oncol.Let. 7:95-100 IHC-P ; Human.
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| 研究领域 | 肿瘤 心血管 细胞生物 神经生物学 干细胞 生长因子和激素 细胞粘附分子 细胞表面分子 细胞分化 血管内皮细胞 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Zebrafish (predicted: Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 129kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TSP-1: 801-900/1170 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq]. Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Similarity: Belongs to the thrombospondin family. Contains 3 EGF-like domains. Contains 1 TSP C-terminal (TSPC) domain. Contains 1 TSP N-terminal (TSPN) domain. Contains 3 TSP type-1 domains. Contains 8 TSP type-3 repeats. Contains 1 VWFC domain. SWISS: P07996 Gene ID: 7057 Database links: Entrez Gene: 100057478 Horse Entrez Gene: 7057 Human Entrez Gene: 21825 Mouse Entrez Gene: 100294615 Sheep Omim: 188060 Human SwissProt: P07996 Human SwissProt: P35441 Mouse Unigene: 164226 Human Unigene: 4159 Mouse Unigene: 185771 Rat 凝血酶敏感蛋白1又称：血小板反应蛋白/凝血酶敏感蛋白1（TSP1）最早发现于血小板中，TSP1在多种组织中广泛表达，并参与细胞黏附、细胞分化和血管新生等多种生物学过程,TSP1也参与血液凝固。 |
| 产品图片 |
Sample:
293T(Human) Cell Lysate at 30 ug Primary: Anti-THBS1 (bs-2715R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kD Observed band size: 129 kD 
Paraformaldehyde-fixed, paraffin embedded (human myocardial tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THBS1) Polyclonal Antibody, Unconjugated (bs-2715R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
