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Rabbit Anti-GSTP1  antibody (bs-2735R)
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2735R
英文名称 GSTP1
中文名称 谷胱甘肽硫转移酶pi抗体
别    名 Glutathione S-Transferase pi; GST-Pi; DFN7; Gst-P1; FAEES3; Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GST3; GSTP1; GST pi; PI; GSTP1_HUMAN.  
Specific References  (6)     |     bs-2735R has been referenced in 6 publications.
[IF=66.85] Fangfei Qin. et al. Linking chromatin acylation mark-defined proteome and genome in living cells. CELL. 2023 Mar;186:1066-1085.e36  Protein purification ;  
[IF=7.83] Zhang, Chunlei, et al. "Insights into the Distinguishing Stress-induced Cytotoxicity of Chiral Gold Nanoclusters and the Relationship with GSTP1." Theranostics  WB ;  Human.  
[IF=6.158] Jiang et al. Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis. (2018) PLoS.Pathog. 14:e1007347  WB ;  Mouse.  
[IF=3.15] Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014).  WB ;  Mouse.  
[IF=2.766] Lin et al. Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury. (2015) PLoS.One. 10:e0138051  WB ;  Rat.  
[IF=2.66] Zhang, Fu, et al. "Up-regulation of protein tyrosine nitration in methamphetamine-induced neurotoxicity through DDAH/ADMA/NOS pathway." Neurochemistry International (2013).  WB ;  Rat.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat)
产品应用 IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 23kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GstP1: 121-210/210 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. Members of the murine GSTP (glutathione S-transferase pi) family, termed Gstp1 and Gstp2, are linked to drug resistance and are markers for many cancers. Gstp proteins modulate cell signaling by interacting with c-Jun N-terminal kinase (JNK), and may play a protective role in the development of spontaneous tumors. Gstp has been found in substantia nigra and may be associated with reactive oxygen species-induced neurological disorders such as Parkinson’s disease and may additionally protect against endothelial dysfunction induced by tobacco smoke exposure.

Function:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Subunit:
Homodimer.

Tissue Specificity:
Ubiquitously expressed.

Similarity:
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.

SWISS:
P19157

Gene ID:
2950

Database links:

Entrez Gene: 2950 Human

Omim: 134660 Human

SwissProt: P09211 Human

Unigene: 523836 Human



GST-Pi是GST家族中的一种主要的同工酶,广泛存在于全身器官中,尤以肝肾含量高,具有解毒功能。在多种肿瘤中高表达。目前认为是肿瘤细胞产生耐药的一种标记,与肿瘤的耐药(阿霉素、顺铂、氮芥、环磷酰胺和瘤可宁等)有关。该抗体主要用于细胞耐药方面的研究。
产品图片
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GSTP1 Polyclonal Antibody, Unconjugated(bs-2735R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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