| 产品编号 | bs-2735R |
| 英文名称 | GSTP1 Rabbit pAb |
| 中文名称 | 谷胱甘肽硫转移酶pi抗体 |
| 别 名 | Glutathione S-Transferase pi; GST-Pi; DFN7; Gst-P1; FAEES3; Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GST3; GSTP1; GST pi; PI; GSTP1_HUMAN. |
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Specific References (6) | bs-2735R has been referenced in 6 publications.
[IF=66.85] Fangfei Qin. et al. Linking chromatin acylation mark-defined proteome and genome in living cells. CELL. 2023 Mar;186:1066-1085.e36 Protein purification ;
[IF=7.83] Zhang, Chunlei, et al. "Insights into the Distinguishing Stress-induced Cytotoxicity of Chiral Gold Nanoclusters and the Relationship with GSTP1." Theranostics WB ; Human.
[IF=6.158] Jiang et al. Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis. (2018) PLoS.Pathog. 14:e1007347 WB ; Mouse.
[IF=3.15] Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014). WB ; Mouse.
[IF=2.766] Lin et al. Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury. (2015) PLoS.One. 10:e0138051 WB ; Rat.
[IF=2.66] Zhang, Fu, et al. "Up-regulation of protein tyrosine nitration in methamphetamine-induced neurotoxicity through DDAH/ADMA/NOS pathway." Neurochemistry International (2013). WB ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GstP1: 121-210/210 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. Members of the murine GSTP (glutathione S-transferase pi) family, termed Gstp1 and Gstp2, are linked to drug resistance and are markers for many cancers. Gstp proteins modulate cell signaling by interacting with c-Jun N-terminal kinase (JNK), and may play a protective role in the development of spontaneous tumors. Gstp has been found in substantia nigra and may be associated with reactive oxygen species-induced neurological disorders such as Parkinson’s disease and may additionally protect against endothelial dysfunction induced by tobacco smoke exposure. Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Subunit: Homodimer. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the GST superfamily. Pi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. SWISS: P09211 Gene ID: 2950 Database links: Entrez Gene: 2950 Human Omim: 134660 Human SwissProt: P09211 Human Unigene: 523836 Human GST-Pi是GST家族中的一种主要的同工酶，广泛存在于全身器官中，尤以肝肾含量高，具有解毒功能。在多种肿瘤中高表达。目前认为是肿瘤细胞产生耐药的一种标记，与肿瘤的耐药（阿霉素、顺铂、氮芥、环磷酰胺和瘤可宁等）有关。该抗体主要用于细胞耐药方面的研究。 |
| 产品图片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GSTP1 Polyclonal Antibody, Unconjugated(bs-2735R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
