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Rabbit Anti-KLF2  antibody (bs-2772R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2772R
英文名称 KLF2
中文名称 肺Kruppel样因子抗体
别    名 Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung); Lung krueppel like factor; Lung Kruppel like zinc finger transcription factor; KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Lklf; Lung krueppel like factor; Lung krueppel-like factor; Lung Kruppel like zinc finger transcription factor.  
Specific References  (7)     |     bs-2772R has been referenced in 7 publications.
[IF=6.691] Cao, Yaochen. et al. Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis. CELL BIOL TOXICOL. 2022 May;:1-17  WB,IHC ;  Mouse.  
[IF=6.548] Wang, Yiying. et al. mTOR contributes to endothelium-dependent vasorelaxation by promoting eNOS expression and preventing eNOS uncoupling. COMMUN BIOL. 2022 Jul;5(1):1-14  WB ;  Human.  
[IF=5.314] Yanda Zhang. et al. Free fatty acids induce coronary microvascular dysfunction via inhibition of the AMPK/KLF2/eNOS signaling pathway. INT J MOL MED. 2023 Apr;51(4):1-14  IHC,WB ;  Mouse.  
[IF=3.968] Hong Jin. et al. BDNF corrects NLRP3 inflammasome-induced pyroptosis and glucose metabolism reprogramming through KLF2/HK1 pathway in vascular endothelial cells. Cell Signal. 2021 Feb;78:109843  WB ;  Human.  
[IF=3.53] Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D (2015) Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE 10(7): e0134390.  IHC-F ;  Mouse.  
[IF=2.99] Wang, Y. L., et al. "Effects of Artesunate and Ursolic Acid on Hyperlipidemia and its Complications in Rabbit." European Journal of Pharmaceutical Sciences (2013).  WB ;  Rabbit.  
[IF=2.1] Xiaoyan Hu. et al. Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Mol Med Rep. 2020 Sep;22(3):2003-2011  WB ;  Rat.  
研究领域 肿瘤  细胞生物  信号转导  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Pig,Cow,Horse,Rabbit,Sheep)
产品应用 WB=1:100-500, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 37kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KLF2: 251-355/355 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]

Function:
Binds to the CACCC box in the beta-globin gene promoter and activates transcription .

Subunit:
Interacts with WWP1.

Subcellular Location:
Nucleus.

Post-translational modifications:
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
Q9Y5W3

Gene ID:
10365

Database links:

Entrez Gene: 10365 Human

Entrez Gene: 16598 Mouse

Entrez Gene: 306330 Rat

Omim: 602016 Human

SwissProt: Q9Y5W3 Human

SwissProt: Q60843 Mouse

SwissProt: Q9ET58 Rat

Unigene: 107740 Human

Unigene: 726356 Human

Unigene: 26938 Mouse

Unigene: 92653 Rat



产品图片
Sample:
Lane1:Brain (Mouse) Lysate at 30 ug
Lane2:HepG2 Cell Lysate at 30 ug
Primary: Anti-KLF2 (bs-2772R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :39 kD
Observed band size :39 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (bs-2772R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (bs-2772R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated(bs-2772R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-KLF2 antibody (bs-2772R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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