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Rabbit Anti-prox1 antibody (bs-2774R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-2774R
英文名称 prox1
中文名称 prox-1抗体
别    名 Homeobox prospero like protein; Homeobox prospero like protein PROX1; Prospero related homeobox 1;prox-1; PROX 1.  
Specific References  (3)     |     bs-2774R has been referenced in 3 publications.
[IF=1.347] Teng,et al.Anti-inflammatory effect of tranexamic acid against trauma-hemorrhagic shock-induced acute lung injury in rats.(2018) Experimental Animals. :.  WB ;  Rat.  
[IF=1.918] Gao,et al.High Expression of Prospero-Related Homeobox-1 (PROX1) Is Associated With Poor Prognosis in Patients With Salivary Adenoid Cystic Carcinoma.(2018) Journal of Oral and Maxillofacial Surgery. :.  IHC-P ;  Human.  
[IF=1.61] Usui, Tatsuya, et al. "Establishment of a novel three‐dimensional primary culture model for hippocampal neurogenesis." Physiological Reports 5.12 (2017): e13318.  IF(IHC-P) ;  Mouse.  
研究领域 心血管  免疫学  神经生物学  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 ELISA=1:2000-5000 IHC-P=1:100-500 Flow-Cyt=2ug/Test (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 81kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human prox-1:151-250/737 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Prox1 is a prospero-related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and the development of postmitotic undifferentiated young neurons.

Subcellular Location:
Nucleus.

Tissue Specificity:
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.

SWISS:
Q92786

Gene ID:
5629

Database links:

Entrez Gene: 395802 Chicken

Entrez Gene: 5629 Human

Entrez Gene: 19130 Mouse

Entrez Gene: 305066 Rat

Entrez Gene: 399348 Xenopus laevis

Entrez Gene: 30679 Zebrafish

Omim: 601546 Human

SwissProt: Q91018 Chicken

SwissProt: Q92786 Human

SwissProt: P48437 Mouse

Unigene: 741808 Human

Unigene: 744931 Human

Unigene: 132579 Mouse

Unigene: 392678 Mouse

Unigene: 26557 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

prox-1主要表达于晶状体、心脏、肝脏、胰腺和神经系统等的非内皮细胞,在成人正常组织、胚胎淋巴管内皮细胞或肿瘤组织的淋巴内皮细胞上也可检测到。移除prox-1基因后,胚胎发育时原始静脉内皮细胞生成淋巴管过程被阻断,表明prox-1是淋巴管生成最基本的调控因子.
产品图片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-prox1 Polyclonal Antibody, Unconjugated(bs-2774R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-prox1 antibody (bs-2774R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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