| 产品编号 | bs-2240R |
| 英文名称 | MYL3 |
| 中文名称 | 肌球蛋白轻链3抗体 |
| 别 名 | Cardiac myosin light chain 1; Slow skeletal Myosin; CMH8; CMLC1; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B ventricular isoform; myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular slow twitch myosin alkali light chain; Ventricular/slow twitch myosin alkali light chain; VLC1. |
| 研究领域 | 心血管 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse (predicted: Rabbit,Cow,Pig,Dog,Chicken,Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:1000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3: 101-195/195 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker. Function: Regulatory light chain of myosin. Does not bind calcium. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Post-translational modifications: The N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1. DISEASE: Cardiomyopathy, familial hypertrophic 8 (CMH8)[MIM:608751]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Rarely, patients present a variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 3 EF-hand domains. SWISS: P08590 Gene ID: 4634 Database links: Entrez Gene: 4634 Human Entrez Gene: 17897 Mouse Omim: 160790 Human SwissProt: P08590 Human SwissProt: P09542 Mouse Unigene: 517939 Human Unigene: 7353 Mouse Unigene: 1955 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Muscle tissue lysates Lane 2: Rat Muscle tissue lysates Primary: Anti-MYL3 (bs-2240R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 25 kDa 
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(bs-2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(bs-2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
