| 产品编号 | bs-3196R |
| 英文名称 | Phospho-IRF7 (Ser471 + Ser472) |
| 中文名称 | 磷酸化干扰素调节因子7抗体 |
| 别 名 | Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H. |
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Specific References (8) | bs-3196R has been referenced in 8 publications.
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472: GV(p-S)(p-S)LD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008] Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus. Tissue Specificity: Expressed predominantly in spleen, thymus and peripheral blood leukocytes. Post-translational modifications: In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. TRAF6-mediated ubiquitination is required for IRF7 activation. Similarity: Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain. SWISS: Q92985 Gene ID: 3665 Database links: Entrez Gene: 3665 Human Entrez Gene: 54123 Mouse Omim: 605047 Human SwissProt: Q92985 Human SwissProt: P70434 Mouse Unigene: 166120 Human Unigene: 3233 Mouse Unigene: 101159 Rat |
| 产品图片 |
Sample:
Uterus (Mouse) Lysate at 40 ug Large intestine (Mouse) Lysate at 40 ug Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD 
Sample:
Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Mouse Blood cell lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Spleen tissue lysates Lane 6: Rat Liver tissue lysates Lane 7: Human Jurkat cell lysates Lane 8: Human Raji cell lysates Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (bs-3196R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 70,55 kD 
Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 48kD 
Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD 
Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (bs-3196R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 54kD Observed band size : 51kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) polyclonal Antibody, Unconjugated (bs-3196R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
