产品编号 | bs-3461R |
英文名称 | Phospho-PPIG (Ser376) |
中文名称 | 磷酸化亲环蛋白(亲环素)PPIG抗体 |
别 名 | CARS cyclophilin; CARS Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Rotamase G; RotamaseG; PPIG_HUMAN. |
产品类型 | 磷酸化抗体 |
研究领域 | 细胞生物 免疫学 染色质和核信号 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Sheep) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 89kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PPIG around the phosphorylation site of Ser376: VS(p-S)GE |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
PPIG belongs to the the peptidyl-prolyl cis/trans isomerase protein family (PPIases). PPIases accelerate the folding of proteins. PPIG catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is distributed in nuclear speckles, a nuclear compartment rich in splicing factors, and colocalizes with the splicing factors SC35 and pinin, suggesting a role in the regulation of pre-mRNA splicing. Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing. Subunit: Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II. Subcellular Location: Nucleus matrix. Nucleus speckle. Note=Colocalizes with RNA splicing factors at nuclear speckles. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 PPIase cyclophilin-type domain. SWISS: Q13427 Gene ID: 9360 Database links: Entrez Gene: 9360 Human Entrez Gene: 228005 Mouse Omim: 606093 Human SwissProt: Q13427 Human SwissProt: A2AR02 Mouse Unigene: 727580 Human Unigene: 11815 Mouse Unigene: 474951 Mouse Unigene: 10898 Rat 肽基脯氨酰基顺反异构酶,广泛存在于各种组织和器官。根据对免疫抑制剂的敏感性,分属三个独立的家族:亲环蛋白PPIG、FK506结合蛋白和细蛋白。能够加速自身折叠和体内新生肽链折叠及变性蛋白质的体外折叠,主要分布于内质网。 |
产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-PPIG (Ser376) (bs-3461R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 89 kD Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPIG (Ser376)) Polyclonal Antibody, Unconjugated (bs-3461R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |