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Rabbit Anti-DMT1  antibody (bs-3577R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-3577R
英文名称 DMT1
中文名称 金属离子转运体1抗体
别    名 DCT 1; DCT1; Divalent metal transporter 1; DMT 1; Natural resistance associated macrophage protein 2; NRAMP 2; NRAMP2; SLC11A2; NRAM2_HUMAN.  
Specific References  (8)     |     bs-3577R has been referenced in 8 publications.
[IF=10.787] Limin Shi. et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway. REDOX BIOL. 2023 Jul;63:102727  WB ;  Mouse.  
[IF=6.543] Zhang Hongxia. et al. Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage. Oxid Med Cell Longev. 2021;2021:9800794  WB,IF ;  Rat.  
[IF=6.17] Qian Wang. et al. Efficient iron utilization compensates for loss of extracellular matrix of ovarian cancer spheroids. Free Radical Bio Med. 2021 Feb;164:369  IF ;  Human.  
[IF=5.959] Zhang C et al. “Iron free” zinc oxide nanoparticles with ion-leaking properties disrupt intracellular ROS and iron homeostasis to induce ferroptosis. Cell Death Dis. 2020 Mar 13;11(3):183.  WB ;  mouse.  
[IF=3.7] Zhang, Ying, et al. "Calcium channel blockers ameliorate iron overload-associated hepatic fibrosis by altering iron transport and stellate cell apoptosis." Toxicology and Applied Pharmacology (2016).  WB ;  Mouse.  
[IF=3.378] Song, Yanqiu. et al. Iron overload impairs renal function and is associated with vascular calcification in rat aorta. BIOMETALS. 2022 Sep;:1-15  IF ;  Rat.  
[IF=2.1] Zhang, L. Y., et al. "Effect of iron source on iron absorption and gene expression of iron transporters in the ligated duodenal loops of broilers." Journal of Animal Science (2017).  WB ;  Chicken.  
[IF=0] Lan B et al. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine. 2020.  IHC-P ;  Rat.  
研究领域 肿瘤  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Cow)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 62kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DMT1: 251-350/568 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 DMT1 is a major transporter of ferrous iron from the intestinal lumen. DMT1 belongs to the family of NRAMP metal ion transporters which function in diverse organisms from bacteria to human. DMT1 (NRAMP2) transports metal ions at the plasma membrane of cells of both the duodenum and in peripheral tissues, and defective DMT1 causes anaemia.

Function:
Actin-bundling protein. May function in mitogen-activated protein kinase pathway.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Heart, brain, lung, skeletal muscle, and kidney.

Post-translational modifications:
Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.

Similarity:
Belongs to the villin/gelsolin family.
Contains 1 HP (headpiece) domain.

SWISS:
P49281

Gene ID:
4891

Database links:

Entrez Gene: 4891 Human

Entrez Gene: 18174 Mouse

Omim: 600523 Human

SwissProt: P49281 Human

SwissProt: P49282 Mouse

Unigene: 505545 Human

Unigene: 234608 Mouse



产品图片
Sample:
Adrenal glands (Mouse) Lysate at 40 ug
Primary: Anti-DMT1 (bs-3577R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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