| 产品编号 | bs-3654R |
| 英文名称 | FPRL1 Rabbit pAb |
| 中文名称 | 脂氧素受体1抗体 |
| 别 名 | ALXR; FMLP R I; FMLP R II; FMLP related receptor I; FMLP-R-I; FMLP-R-II; FMLP-related receptor I; FMLPX; Formyl peptide receptor 2; Formyl peptide receptor like 1; Formyl peptide receptor related; Formyl peptide receptor-like 1; FPR 2; FPR2; FPR2_HUMAN; FPR2A; FPRH 1; FPRH 2; FPRH1; FPRH2; FPRL 1; FPRL1; HM63; Lipoxin A4 receptor; LXA4 receptor; LXA4R; N-formyl peptide receptor 2; RFP. |
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Specific References (4) | bs-3654R has been referenced in 4 publications.
[IF=8.702] Cao Yirui. et al. Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemia–reperfusion injury. CELL MOL BIOL LETT. 2023 Dec;28(1):1-24 FCM ; Rat.
[IF=5.23] Diao, Na, et al. "Deficiency in Toll-interacting protein (Tollip) skews inflamed yet incompetent innate leukocytes in vivo during DSS-induced septic colitis."Scientific Reports 6 (2016): 34672. WB ; Mouse.
[IF=4.966] Wenzheng Xia. et al. ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway. Faseb J. 2020 Oct;34(10):13993-14005 IHC ; Rat.
[IF=4.26] Wang et al. Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells. (2016) Sci.Re. 6:25946 WB, IF(ICC) ; Rat.
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| 研究领域 | 心血管 细胞生物 免疫学 信号转导 细胞粘附分子 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FPRL1: 51-150/351 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FPRL1 is a low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). FPRL1 has been reported in phagocytes, monocytes, neutrophils, differentiated myeloid cells from bone marrow, granulocyte HL-60 cells, and synovial fibroblasts. ESTs have been isolated from blood, leukocyte, lung, and placenta libraries. Function: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25090 Gene ID: 2358 Database links: Entrez Gene: 2358 Human Omim: 136538 Human SwissProt: P25090 Human Unigene: 99855 Human 脂氧素受体广泛表达于各类炎症相关细胞并受多种炎症刺激的调节,通过PKA、PKC、MAPK等一系列信号途径参与控制炎症的发生发展。 |
| 产品图片 |
Sample:
SW480(Human) Cell Lysate at 30 ug
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-FPRL1 (bs-3654R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Sample:Liver(Mouse) Lysate at 40 ug
Primary: Anti-FPRL1(bs-3654R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 47 kD
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- FPRL1 (bs-3654R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Sample:
Lane 1: U937 (Human) Cell Lysate at 30 ug
Lane 2: HL60 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: SW480 (Human) Cell Lysate at 30 ug
Lane 6: Lovo (Human) Cell Lysate at 30 ug
Primary: Anti-FPRL1 (bs-3654R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 35 kD
Sample:
kidney (Mouse) Lysate at 40 ug
Primary: Anti-FPRL1 (Bs-3654R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 48 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
