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Rabbit Anti-BRN3A  antibody (bs-3669R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-3669R
英文名称 BRN3A
中文名称 转录因子Brn3蛋白抗体
别    名 Brain specific homeobox/POU domain protein 3A; Brn 3.0; BRN 3A; Brn3; Homeobox/POU domain protein RDC 1; Homeobox/POU domain protein RDC1; Oct T1; OctT1; POU Domain Class 4 Transcription Factor 1; POU4F1; RDC 1; RDC1.  Brn3α;
Specific References  (7)     |     bs-3669R has been referenced in 7 publications.
[IF=17.839] Xue, Yuanchao, et al. "Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells." Nature Neuroscience (2016).  IF(ICC) ;  Human.  
[IF=10.19] Zhuhe Liu. et al. CXCL7 aggravates the pathological manifestations of neuromyelitis optica spectrum disorder by enhancing the inflammatory infiltration of neutrophils, macrophages and microglia. CLIN IMMUNOL. 2022 Sep;:109139  IF ;  Mouse.  
[IF=5.988] Yifan Song. et al. Matrine promotes mitochondrial biosynthesis and reduces oxidative stress in experimental optic neuritis. FRONT PHARMACOL. 2022; 13: 936632  IF ;  Rat.  
[IF=3.84] Bell, Katharina, et al. "Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture." Journal of Neurochemistry (2016).  IHC-P ;  Pig.  
[IF=3.77] Siqi Li. et al. Structural damage to the rat eye following long-term simulated weightlessness. EXP EYE RES. 2022 Oct;223:109200  IF ;  Rat.  
[IF=3.467] Sujia Ma. et al. Effect of papaverine on axonal outgrowth of primary retinal ganglion cells of Sprague Dawley rats. Exp Eye Res. 2021 Oct;:108797  IF ;  Rat .  
[IF=1.652] Wang F et al. Gypenosides attenuate lipopolysaccharide-induced optic neuritis in rats. Acta Histochem. 2018 May;120(4):340-346.  IF ;  Rat.  
研究领域 免疫学  神经生物学  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 43kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BRN3A: 325-419/419 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012].

Function:
Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.

Subcellular Location:
Nucleus.

Tissue Specificity:
Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.

DISEASE:
Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.

Similarity:
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

SWISS:
Q01851

Gene ID:
5457

Database links:

Entrez Gene: 5457 Human

Entrez Gene: 18996 Mouse

Entrez Gene: 114503 Rat

Omim: 601632 Human

SwissProt: Q01851 Human

SwissProt: P17208 Mouse

SwissProt: P20266 Rat

Unigene: 654522 Human

Unigene: 246550 Mouse

Unigene: 232568 Rat



转录因子Brn3可能直接参与对轴突和树突生长能力的调控。
产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-BRN3A (bs-3669R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 33 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-0071R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-BRN3A antibody (bs-3669R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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