产品编号 | bs-3669R |
英文名称 | BRN3A |
中文名称 | 转录因子Brn3蛋白抗体 |
别 名 | Brain specific homeobox/POU domain protein 3A; Brn 3.0; BRN 3A; Brn3; Homeobox/POU domain protein RDC 1; Homeobox/POU domain protein RDC1; Oct T1; OctT1; POU Domain Class 4 Transcription Factor 1; POU4F1; RDC 1; RDC1. Brn3α; |
Specific References (7) | bs-3669R has been referenced in 7 publications.
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研究领域 | 免疫学 神经生物学 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Rabbit) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 43kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BRN3A: 325-419/419 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]. Function: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Subcellular Location: Nucleus. Tissue Specificity: Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye. DISEASE: Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth. Similarity: Belongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q01851 Gene ID: 5457 Database links: Entrez Gene: 5457 Human Entrez Gene: 18996 Mouse Omim: 601632 Human SwissProt: Q01851 Human SwissProt: P17208 Mouse Unigene: 654522 Human Unigene: 246550 Mouse Unigene: 232568 Rat 转录因子Brn3可能直接参与对轴突和树突生长能力的调控。 |
产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-BRN3A (bs-3669R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 33 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-0071R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-BRN3A antibody (bs-3669R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |