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Rabbit Anti-TGF beta 1 antibody (bs-0086R)
~~~促销,代码SSY221101~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0086R
英文名称 TGF beta 1
中文名称 转化生长因子β1/TGF β1/TGF-β1抗体
别    名 CED; DPD1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1.  
Specific References  (65)     |     bs-0086R has been referenced in 65 publications.
[IF=3.269] Ji-Xian Song. et al. Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice. Bioengineered. 2022;13(4):8334-8348  IHC ;  Mouse.  
[IF=1.88] Venter, Neil Grant, et al. "Use of platelet-rich plasma in deep second-and third-degree burns." Burns (2016).  other ;  
[IF=5.29] Shang, Peijin, et al. "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." Scientific Reports 6 (2016): 39809.  IHC-P ;  Rat.  
[IF=1.51] Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52  IHC ;  Rat.  
[IF=4.679] Guannan Leet al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol . 2020 Sep;143:111516.  WB ;  Human.  
[IF=2.311] Zhao Y et al. Interaction between regulatory T cells and mast cells via IL‑9 and TGF‑β productionOncol Lett.2020 Dec;20(6):360.  IF ;  Human.  
[IF=4.545] Yi Zhang. et al. Nanoparticle conjugation of ginsenoside Rb3 inhibits myocardial fibrosis by regulating PPARα pathway. Biomed Pharmacother. 2021 Jul;139:111630  WB ;  Rat.  
[IF=5.682] Xiaolan You. et al. Dihydroartemisinin attenuates pulmonary inflammation and fibrosis in rats by suppressing JAK2/STAT3 signaling. Aging-Us. 2022 Feb 15; 14(3): 1110–1127  IHC,WB ;  Rat.  
[IF=4.164] Chengnan Tian. et al. CREB1 transcription-activated lncRNA PVT1 promotes cardiac fibrosis via miR-145/HCN1 axis. Int J Cardiol. 2022 Jan;:  WB ;  Mouse,Human.  
[IF=5.396] Yan Zhang. et al. Polysaccharide from Ganoderma lucidum Alleviates Congnitive Impairment in a Mice Model of Chronic Cerebral Hypoperfusion by Regulating CD4+CD25+Foxp3+ regulatory T cells. Food Funct. 2022 Jan;:  WB ;  Mouse.  
[IF=6.291] Wei Cui. et al. Cadmium exposure activates the PI3K/AKT signaling pathway through miRNA-21, induces an increase in M1 polarization of macrophages, and leads to fibrosis of pig liver tissue. Ecotox Environ Safe. 2021 Dec;228:113015  WB ;  Pig.  
[IF=5.279] Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204–14214  WB ;  Mouse.  
[IF=2.606] Lei Zhang. et al. Autologous bone marrow-derived mesenchymal stem cells for interstitial fibrosis and tubular atrophy: a pilot study. Renal Failure. 2021;43(1):1266-1275  IHC ;  human.  
[IF=6.048] Qingqing Liu. et al. Probiotics alleviate autoimmune hepatitis in mice through modulation of gut microbiota and intestinal permeability. J Nutr Biochem. 2021 Sep;:108863  IHC ;  mosue.  
[IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  WB ;  Mouse.  
[IF=5.682] Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464–18481  WB,IHC ;  Mouse.  
[IF=3.118] Wen-Jin Chen. et al. The Role of Small Extracellular Vesicles Derived from Lipopolysaccharide Preconditioned hDPSCs in Dental Pulp Regeneration. J Endodont. 2021 Mar;:  IHC ;  Rat.  
[IF=2.476] Yu Shan. et al. miR-98-5p protects against cerebral ischemia/reperfusion injury through anti-apoptosis and anti-oxidative stress in mice. J Biochem. 2021 Mar;169(2):195-206  WB ;  Human.  
[IF=2.1] Zhenjie Huang. et al. Protective effects of different anti‑inflammatory drugs on tracheal stenosis following injury and potential mechanisms. Mol Med Rep. 2021 May;23(5):1-11  WB ;  Rabbit.  
[IF=4.545] Xiaoxi Cheng. et al. Hair follicle bulge-derived stem cells promote tissue regeneration during skin expansion. Biomed Pharmacother. 2020 Dec;132:110805  WB ;  Rat.  
[IF=4.171] Yi Chen. et al. The essential oil from the raw and vinegar processed Rhizoma Curcumae ameliorate CCl4-incuded liver fibrosis: integrating network pharmacology and molecular mechanism evaluation. 2021 Mar 17  WB ;  Rat.  
[IF=3.69] Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275  WB ;  Rat.  
[IF=1.27] Tingting Cao. et al. Effects of Salvia miltiorrhiza and Radix astragali on the TGF-β/Smad/Wnt pathway and the pathological process of liver fibrosis in rats. Cell Mol Biol. 2020 Sep;66(6):46-51  WB ;  Rat.  
[IF=3.915] Xinlu Zhang. et al. Identification of Transcriptional Variation in Aortic Remodeling Using a Murine Transverse Aortic Constriction (TAC) Model.. Front Cardiovasc Med. 2020; 7: 581362  WB,IF,IHC ;  Mouse.  
[IF=3.69] Yuling Tong. et al. Zuojin Pill ameliorates chronic atrophic gastritis induced by MNNG through TGF-β1/PI3K/Akt axis. J Ethnopharmacol. 2021 May;271:113893  WB ;  Rat.  
[IF=7.577] Wei C et al. Nanocolloids in drinking water increase the risk of obesity in mice by modulating gut microbesEnviron Int.2021 Jan;146:106302.  IHC ;  Mouse.  
[IF=4.152] Wang B et al. Preparation of Fibroblast Suppressive Poly(ethylene glycol)-b-poly(l-phenylalanine)/Poly(ethylene glycol) Hydrogel and Its Application in Intrauterine Fibrosis Prevention  IHC ;  Rat.  
[IF=4.679] Le G et al. Ochratoxin A Induces Glomerular Injury Through Activating the ERK/NF-κB Signaling Pathway. Food Chem Toxicol. 2020 Jun 29;111516.  WB,IF ;  human.  
[IF=3.571] Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;68(15):4399-4410.  IHC-P ;  Rat.  
[IF=0] Afdal A et al. The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer. Open Access Maced J Med Sci. 2019 Jun 30;7(12):1905-1910.  IHC-P ;  Human.  
[IF=3.367] Liu M et al. The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro. Cell Mol Biol Lett. 2019 Jun 22;24:44.  WB ;  Deer.  
[IF=2.884] Li HT et al. CpG-ODNs and Budesonide Act Synergistically to Improve Allergic Responses in Combined Allergic Rhinitis and Asthma Syndrome Induced by Chronic Exposure to Ovalbumin by Modulating the TSLP-DC-OX40L Axis.Inflammation. 2018 Aug;41(4):1304-1320.  IHC ;  Mouse.  
[IF=5.5] Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-306  WB ;  Rabbit.  
[IF=1.92] Wang et al. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. (2018) Mol.Med.Rep. 18:3143-3152  WB ;  Rat.  
[IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.  IHC-P ;  Mouse.  
[IF=2.13] Mao, Rui, et al. "Efficacy of radiotherapy for the treatment of cystic echinococcosis in naturally infected sheep." Infectious Diseases of Poverty 6.1 (2017): 88.  IHC-P ;  Sheep.  
[IF=1.89] Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525.  IHC-P ;  Mouse.  
[IF=3.23] Xue X, Chen Q, Zhao G, Zhao J-Y, Duan Z, Zheng P-S (2015) The Overexpression of TGF-β and CCN2 in Intrauterine Adhesions Involves the NF-κB Signaling Pathway. PLoS ONE 10(12): e0146159.  IHC-P ;  Human.  
[IF=1.27] Sun, Zhenqiang, et al. "Immunological effect induced by mesenchymal stem cells in a rat liver transplantation model." Experimental and therapeutic medicine 10.2 (2015): 401-406.  IHC-P ;  Rat.  
[IF=7.39] Ganguly, Rituparna, et al. "Anti-atherogenic Effect of Trivalent Chromium-loaded CPMV Nanoparticles in Human Aortic Smooth Muscle Cells under Hyperglycemic Conditions in vitro." Nanoscale (2016).  WB ;  Human.  
[IF=1.64] Eren, Kenan, et al. "The Suppression of Wound Healing Response with Sirolimus and Sunitinib Following Experimental Trabeculectomy in a Rabbit Model." Current Eye Research (2016): 1-10.  IHC-P ;  Rabbit.  
[IF=0.94] Tang, Jin, et al. "Increased adenosine levels contribute to ischemic kidney fibrosis in the unilateral ureteral obstruction model." Experimental and Therapeutic Medicine.  IHC-P ;  Mouse.  
[IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.  WB ;  Mouse.  
[IF=1.879] Baojian Wang. et al. The increased motion of lumbar induces ligamentum flavum hypertrophy in a rat model. Bmc Musculoskel Dis. 2021 Dec;22(1):1-9  IHC ;  Rat.  
[IF=4.36] An Wang. et al. Yiqi Jiedu herbal decoction attenuates the 2 Gy 60Co γ ray induced spleen injury by inhibiting apoptosis and modulating the immune balance. J Ethnopharmacol. 2022 Mar;286:114925  IHC ;  Mouse.  
[IF=4.23] Zhu, Boqian. et al. Protective effect of rosuvastatin against the formation of benign esophageal stricture. 2021 Nov 20  IHC ;  Rabbit.  
[IF=5.241] Wang, Lingfang. et al. miR-2337 induces TGF-β1 production in granulosa cells by acting as an endogenous small activating RNA. Cell Death Discov. 2021 Sep;7(1):1-9  WB ;   Porcine.  
[IF=5.923] Kun-Ling Lin. et al. Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial. Int J Mol Sci. 2021 Jan;22(17):9296  WB ;  Rat.  
[IF=4.171] Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brain–liver axis in rats. 2021 May 18  WB ;  Rat.  
[IF=1.898] Yong-Chang Duan. et al. Swimming Exercise Ameliorates Hypertension-Induced Kidney Dysfunction via Alleviating Renal Interstitial Fibrosis and Apoptosis. Kidney Blood Press R. 2021;46(2):219-228  WB ;  Rat.  
[IF=1.785] Ahmet Türkeli. et al. Anti‑VEGF treatment suppresses remodeling factors and restores epithelial barrier function through the E‑cadherin/β‑catenin signaling axis in experimental asthma models. Exp Ther Med. 2021 Jul;22(1):1-9  IHC ;  Mouse.  
[IF=9.038] Bing Han. et al. Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway. J Hazard Mater. 2021 May;:125984  WB ;  Mouse.  
[IF=3.69] Qingqing Chen. et al. Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity. J Ethnopharmacol. 2021 Mar;267:113542  IHC ;  Rat.  
[IF=1.813] Zhao Hong. et al. Study on Network Pharmacological Analysis and Preliminary Validation to Understand the Mechanisms of Plantaginis Semen in Treatment of Gouty Nephropathy. Evid-Based Compl Alt. 2020;2020:8861110  IHC ;  Rat.  
[IF=4.545] Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-β1/Smad3 pathwayBiomed Pharmacother.2020 Dec 24;134:111135.  IF、WB ;  Mouse.  
[IF=1.96] Kou, Wei, et al. ʺTransforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment.ʺ Inflammation Research (2013): 1-7.  ELISA ;  Human.  
[IF=1.813] Li Y et al. Study on the Anticancer Effect of an Astragaloside- and Chlorogenic Acid-Containing Herbal Medicine (RLT-03) in Breast Cancer. Evid Based Complement Alternat Med. 2020 Jun 10;2020:1515081.  IHC-P ;  Human.  
[IF=4.679] Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516.  WB&ICF ;  Human.  
[IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062.  IHC-P ;  Rat.  
[IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82.  WB ;  Rabbit.  
[IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344  IHC ;  Human.  
[IF=2.45] Xiu, Zhi-Ming, et al. "1-Acetyl-5-phenyl-1H-pyrrol-3-ylacetate: an aldose reductase inhibitor for the treatment of diabetic nephropathy." Bioorganic & Medicinal Chemistry Letters (2017).  WB ;  Rat.  
[IF=1.4] Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445.  IHC-P ;  Mouse.  
[IF=4.52] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Reduces Bone Formation in Vivo and Osteoblast Differentiation in Vitro." The American Journal of Pathology (2014).  WB ;  Rat.  
[IF=1.06] Erbas, O., et al. "Nephro-protective effect of granulocyte colony-stimulating factor in streptozotocin induced diabetic rats." Biotechnic & Histochemistry 0 (2014): 1-9.  IHC-P ;  Rat.  
研究领域 肿瘤  心血管  信号转导  干细胞  生长因子和激素  转录调节因子  细胞因子  细胞外基质  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 12.8/44kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TGF-Beta 1: 351-390/390 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]

Function:
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Subunit:
Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

Post-translational modifications:
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

DISEASE:
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

Similarity:
Belongs to the TGF-beta family.

SWISS:
P01137

Gene ID:
7040

Database links:

Entrez Gene: 7040 Human

Entrez Gene: 21803 Mouse

Entrez Gene: 59086 Rat

Omim: 190180 Human

SwissProt: P01137 Human

SwissProt: P04202 Mouse

SwissProt: P17246 Rat

Unigene: 645227 Human



生长因子和激素( Growth Factor and Hormones) TGF是一种多效生长因子,对上皮细胞增值有潜在抑制作用,可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。 TGFβ超级家族由为数众多的生长的分化因子组成,包括转移生长因子β1,2和3(TGF β1,TGF β2,TGF β3);胎盘生长因子(PTGF-β);生长/分化因子(GDFs);缪氏抑制物(MIS);骨形态形成蛋白(BMPs);交织细胞元神经生长因子(GDNF);抑制素和活化素(α, β-A,和β-C),Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有交叉反应。与豚鼠有大部分交叉。
产品图片
Sample:
Liver (Mouse) Lysate at 40 ug
Liver (Rat) Lysate at 40 ug
Primary: Anti- TGF beta 1 (bs-0086R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44 kD
Observed band size: 55 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44 kD
Observed band size: 59 kD
Sample:
Embryo (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44 kD
Observed band size: 59 kD
Sample:TGF beta1 recombinant protein (human) Lysate at 100ng
Primary: Anti- TGF beta1 (bs-0086R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44 kD
Observed band size: 12.8 kD
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Placenta (Mouse) Lysate at 40 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Liver (Rat) Lysate at 40 ug
Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/25/12.5 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGF beta 1) Polyclonal Antibody, Unconjugated (bs-0086R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control:MG63.
Primary Antibody (green line): Rabbit Anti-TGF beta 1 antibody (bs-0086R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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