| 产品编号 | bs-3682R |
| 英文名称 | Nox1 Rabbit pAb |
| 中文名称 | 有丝分裂氧化酶1抗体 |
| 别 名 | Mitogenic oxidase 1; MOX1; NADH/NADPH mitogenic oxidase subunit P65 MOX; NADPH oxidase 1; NADPH oxidase 1 variant NOH 1L; NADPH oxidase homolog 1; NOH 1; NOH1; RP1-146H21.1; GP91-2; Mitogenic oxidase(pyridine nucleotide dependent superoxide generating). |
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Specific References (9) | bs-3682R has been referenced in 9 publications.
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| 研究领域 | 免疫学 激酶和磷酸酶 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nox1: 425-515/515 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation. Subunit: NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1. Subcellular Location: Cell projection, invadopodium membrane; Multi-pass membrane protein. Tissue Specificity: NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. SWISS: Q9Y5S8 Gene ID: 27035 Database links: Entrez Gene: 27035 Human Entrez Gene: 237038 Mouse Omim: 300225 Human SwissProt: Q9Y5S8 Human SwissProt: Q8CIZ9 Mouse Unigene: 592227 Human Unigene: 233865 Mouse |
| 产品图片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- Nox1 (bs-3682R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti- Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
