| 产品编号 | bs-3817R |
| 英文名称 | Piwil2 |
| 中文名称 | piwi样2蛋白抗体 |
| 别 名 | PIWL2_HUMAN; Piwi-like protein 2; EC:3.1.26.-; HILI; CT80; mili; PIWIL1L; piwi like RNA-mediated gene silencing 2; Cancer/testis antigen 80 (CT80); |
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Specific References (3) | bs-3817R has been referenced in 3 publications.
[IF=3.64] Shi, Ran-ran, et al. "The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1."?Cancer Immunology, Immunotherapy?(2013): 1-10. WB ; Human.
[IF=3.49] Easley et al. Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model. (2015) Stem.Cell.Re. 14:347-55 IF(ICC) ; Human.
[IF=2.276] Tian Xiaohong. et al. 17-Allylamino-demethoxygeldanamycin Used Alone or in Combination with Sodium Orthovanadate Promotes Apoptosis and Inhibits Invasion of SH-SY5Y Cells by Modulating PIWIL2. Biomed Res Int. 2020;2020:7894712 WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Rabbit,GuineaPig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 107kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Piwil2/Mili: 885-973/973 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] Function: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Subunit: Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Subcellular Location: Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Tissue Specificity: Expressed in adult testis and in most tumors. Post-translational modifications: Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. Similarity: Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain. SWISS: Q8TC59 Gene ID: 55124 Database links: Entrez Gene: 55124 Human Entrez Gene: 57746 Mouse Omim: 610312 Human SwissProt: Q8TC59 Human SwissProt: Q8CDG1 Mouse Unigene: 614809 Human Unigene: 85253 Mouse Unigene: 144968 Rat [PTM]Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. |
| 产品图片 |
Sample: 293T Cell Lysate at 30 ug
Primary: Anti- Piwil2 (bs-3817R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107kD 
Sample:
testis (Mouse) Lysate at 40 ug 293T Cell (Human) Lysate at 30 ug Primary: Anti- piwil2 (bs-3817R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107 kD 
Sample:
Lovo(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/110 kD Observed band size: 110 kD 
Sample:
Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug HepG2 (Human) Cell Lysate at 30 ug Lovo (Human) Cell Lysate at 30 ug 293T (Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/110 kD Observed band size: 110 kD 
Blank control: Mouse spleen cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Piwil2 antibody(bs-3817R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
