| 产品编号 | bs-2375R |
| 英文名称 | CYP3A4 |
| 中文名称 | 细胞色素P450 3A4抗体 |
| 别 名 | CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA4; Cytochrome P450 3A4; Cytochrome P450 family 3 subfamily A polypeptide 4; Cytochrome P450 subfamily IIIA polypeptide 3; Cytochrome P450 subfamily IIIA polypeptide 4; cytochrome P450, family 3, subfamily a, polypeptide 1; glucocorticoid inducible P450; HLP; MGC126680; NF 25; NF25; Nifedipine oxidase; Niphedipine oxidase; P450 III steroid inducible; P450 PCN(PCNC inducible); P450 PCN1; P450C3; P450PCN1; Quinine 3 monooxygenase; Taurochenodeoxycholate 6 alpha hydroxylase; CP3A4_HUMAN. |
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Specific References (3) | bs-2375R has been referenced in 3 publications.
[IF=7.129] Jie Luo. et al. Copper nanoparticles lead to reproductive dysfunction by affecting key enzymes of ovarian hormone synthesis and metabolism in female rats. ECOTOX ENVIRON SAFE. 2023 Apr;254:114704 WB ; Rat.
[IF=6.59] Jun Li. et al. Therapeutic targets and functions of curcumol against COVID-19 and colon adenocarcinoma. FRONT NUTR. 2022; 9: 961697 IHC ; Human.
[IF=5.923] Hanyi Hua. et al. The Intervention and Mechanism of Action for Aloin against Subchronic Aflatoxin B1 Induced Hepatic Injury in Rats. Int J Mol Sci. 2021 Jan;22(21):11620 IHC ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP3A4: 151-250/503 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011] Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide. Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Tissue Specificity: Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). Post-translational modifications: Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). Similarity: Belongs to the cytochrome P450 family. SWISS: P08684 Gene ID: 1576 Database links: Entrez Gene: 1576 Human Omim: 124010 Human SwissProt: P08684 Human Unigene: 728751 Human
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| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-CYP3A4 (bs-2375R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP3A4) Polyclonal Antibody, Unconjugated (bs-2375R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
