| 产品编号 | bs-3789R |
| 英文名称 | Perilipin A |
| 中文名称 | 脂滴包被蛋白Perilipin-A抗体 |
| 别 名 | Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; Perilipin 1; PerilipinA; Perilipin-A; PLIN; PLIN1; PLIN1_HUMAN. |
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Specific References (1) | bs-3789R has been referenced in 1 publications.
[IF=4.05] Stelmanska, Ewa, Sylwia Szrok, and Julian Swierczynski. "Progesterone induced down regulation of hormone sensitive lipase ( Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis." The Journal of Steroid Biochemistry and Molecular Biology (2014). WB ; Rat.
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| 研究领域 | 心血管 免疫学 合成与降解 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Perilipin-1: 85-180/522 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. Function: Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Subunit: Interacts with ABHD5. Subcellular Location: Lipid droplet. Note=Lipid droplet surface-associated. Tissue Specificity: Adipocytes. Post-translational modifications: Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. DISEASE: Defects in PLIN1 are the cause of familial partial lipodystrophy type 4 (FPLD4) [MIM:613877]. FPLD4 is a form of lipodystrophy characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. Similarity: Belongs to the perilipin family. SWISS: O60240 Gene ID: 5346 Database links: Entrez Gene: 5346 Human Entrez Gene: 103968 Mouse Omim: 170290 Human SwissProt: O60240 Human SwissProt: Q8CGN5 Mouse Unigene: 103253 Human Unigene: 254917 Mouse Unigene: 9737 Rat 脂肪细胞中贮存的甘油三酯的水解是人和动物体内精细调节的动态平衡过程，与维持机体能量的动态平衡和代谢正常密切相关。Perilipin A是成熟脂肪细胞中脂滴膜上表达量较多的一种结构和功能蛋白，是多条脂解通路的终末靶点之一，对脂肪细胞的脂肪分解起着关键的调控作用. perilipin可能在脂肪分解调控中起到“分子开关”的作用。蛋白激酶A（PKA）、细胞外信号调节激酶（ERK）等信号转导通路参与了脂肪分解。肿瘤坏死因子仅（TNFα）、过氧化物酶体增殖物激活受体γ（PPAγ）激动剂、瘦素（leptin）均可以影响perilipin的表达。近年来研究，perilipin可通过蛋白酶体途径来调节其蛋白量的表达。脂肪分解调控中的关键蛋白perilipin对2型糖尿病、肥胖、动脉粥样硬化等多种代谢性疾病及心血管疾病相关联。 |
| 产品图片 |
Sample: Thymus (Mouse) Lysate at 40 ug
Primary: Anti-Perilipin A (bs-3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 
Sample:Parotid gland (Mouse) Lysate at 40 ug
Primary: Anti-Perilipin A (bs-3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 
Sample:
MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-Perilipin A (Bs- 3789R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Perilipin A ) Polyclonal Antibody, Unconjugated (bs-3789R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
