| 产品编号 | bs-2353R |
| 英文名称 | CMA1 |
| 中文名称 | 肥大细胞类糜蛋白酶1抗体 |
| 别 名 | CMA1_HUMAN; Chymase; EC:3.4.21.39; CYH; CYM; Mast Cell Chymase; Alpha-chymase; Mast cell protease I; |
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Specific References (6) | bs-2353R has been referenced in 6 publications.
[IF=11.33] Moretti, Silvia, et al. "A mast cell-ILC2-Th9 pathway promotes lung inflammation in cystic fibrosis." Nature Communications 8 (2017): 14017. IHC-P ; Mouse.
[IF=3.73] Zheng J, Wei C-C, Hase N, Shi K, Killingsworth CR, et al. (2014) Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS ONE 9(4): e94732. IHC-P ; Dog.
[IF=3.73] Wang H, Jessup JA, Zhao Z, Da Silva J, Lin M, et al. (2013) Characterization of the Cardiac Renin Angiotensin System in Oophorectomized and Estrogen-Replete mRen2.Lewis Rats. PLoS ONE 8(10):e76992. WB ; Rat.
[IF=3.23] Sansoè, Giovanni, et al. "Role of Chymase in the Development of Liver Cirrhosis and Its Complications: Experimental and Human Data." PLOS ONE11.9 (2016): e0162644. IHC-P ; Human, Rat.
[IF=2.28] Zhao, Zhuo, et al. "GPR30 Decreases Cardiac Chymase/Angiotensin II by Inhibiting Local Mast Cell Number." Biochemical and Biophysical Research Communications (2015). WB, IHC-P ; Rat.
[IF=1.69] Zeng et al. Resveratrol improves prostate fibrosis during progression of urinary dysfunction in chronic prostatitis by mast cell suppression. (2018) Mol.Med.Rep. 17:918-924 WB ; Rat.
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| 研究领域 | 心血管 免疫学 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Sheep,GuineaPig) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CMA1: 28-42/247 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008] Function: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Subcellular Location: Secreted. Cytoplasmic granule. Note=Mast cell granules. Tissue Specificity: Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. Similarity: Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain. SWISS: P23946 Gene ID: 1215 Database links: Entrez Gene: 1215 Human Entrez Gene: 17228 Mouse SwissProt: P23946 Human SwissProt: P21844 Mouse |
| 产品图片 |
Sample:
Lung (Mouse) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti- Mast Cell Chymase (bs-2353R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 23 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
