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Rabbit Anti-ATG5/APG5L antibody (bs-4005R)
~~~促销,代码SSY221101~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-4005R
英文名称 ATG5/APG5L
中文名称 自噬蛋白5/细胞凋亡的特异性蛋白抗体
别    名 ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN.  
Specific References  (11)     |     bs-4005R has been referenced in 11 publications.
[IF=10.588] Jianzhao Liao. et al. Mitochondrial miR-1285 regulates copper-induced mitochondrial dysfunction and mitophagy by impairing IDH2 in pig jejunal epithelial cells. J Hazard Mater. 2022 Jan;422:126899  WB ;  Pig.  
[IF=13.281] Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 20  WB,IF ;  Human; Mouse.  
[IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639.  WB ;  Mouse.  
[IF=3.53] Lang, Fangfang, et al. "Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells." PLOS ONE 10.6 (2015): e0129196.  WB ;  Human.  
[IF=5.64] Chengcheng Zhang. et al. Autophagy Induced by the N-Terminus of the Classic Swine Fever Virus Nonstructural Protein 5A Protein Promotes Viral Replication. Front Microbiol. 2021; 12: 733385  WB ;  Pig.  
[IF=4.939] Tong-Fei Li. et al. Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma. Mol Pharmaceut. 2021;XXXX(XXX):XXX-XXX  WB ;  Pig.  
[IF=3.216] Lijuan Gao # et al. Chlorogenic Acid Alleviates Aβ25-35-Induced Autophagy and Cognitive Impairment via the mTOR/TFEB Signaling Pathway. Drug Des Devel Ther. 2020 May 4;14:1705-1716.  WB ;  Human.  
[IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801  WB ;  Human.  
[IF=3.85] Li, Yue, et al. "Autophagy contributes to 4-Amino-2-Trifluoromethyl-Phenyl Retinate-induced differentiation in human acute promyelocytic leukemia NB4 cells." Toxicology and Applied Pharmacology (2017).  WB ;  Human.  
[IF=2.33] Tang, Qishan, et al. "Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma." Cancer letters 336.2 (2013): 325-337.  WB ;  Human.  
[IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013).  WB ;  
研究领域 肿瘤  细胞生物  免疫学  细胞凋亡  细胞自噬  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 32kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human APG5L: 101-200/275 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy.

Function:
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity).
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Subunit:
The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate.

Subcellular Location:
Cytoplasm. Note=Colocalizes with nonmuscle actin.

Tissue Specificity:
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Post-translational modifications:
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.

Similarity:
Belongs to the ATG5 family.

SWISS:
Q9H1Y0

Gene ID:
9474

Database links:

Entrez Gene: 9474 Human

Entrez Gene: 11793 Mouse

Entrez Gene: 365601 Rat

Omim: 604261 Human

SwissProt: Q9H1Y0 Human

SwissProt: Q99J83 Mouse

SwissProt: Q3MQ06 Rat

Unigene: 486063 Human

Unigene: 22264 Mouse

Unigene: 98385 Rat



产品图片
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-ATG5/APG5 (bs-4005R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ATG5 antibody (bs-4005R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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