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Rabbit Anti-GPX4 antibody (bs-3884R)

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说明书： 50ul 100ul 200ul

50ul/1180.00元

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100ul/1980.00元

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产品编号	bs-3884R
英文名称	GPX4
中文名称	谷胱甘肽过氧化酶4抗体
别名	Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase.
	Specific References (18) \| bs-3884R has been referenced in 18 publications. [IF=18.027] Ke Li. et al. Oxygen Self-Generating Nanoreactor Mediated Ferroptosis Activation and Immunotherapy in Triple-Negative Breast Cancer. ACS NANO. 2023;17(5):4667–4687 WB ; Mouse. PubMed:36861638 [IF=15.153] Xu Chen. et al. Chiral Ruthenium Nanozymes with Self-Cascade Reaction Driven the NO Generation Induced Macrophage M1 Polarization Realizing the Lung Cancer “Cocktail Therapy”. SMALL. 2023 Apr;:2207823 WB ; Mouse. PubMed:37029560 [IF=12.479] Tao Liu. et al. Multifaceted roles of a bioengineered nanoreactor in repressing radiation-induced lung injury. Biomaterials. 2021 Oct;277:121103 WB,IF ; Mouse. PubMed:34478930 [IF=10.753] Lu Yu. et al. Chronic arsenic exposure induces ferroptosis via enhancing ferritinophagy in chicken livers. SCI TOTAL ENVIRON. 2023 May;:164172 WB ; Chicken. PubMed:37201840 [IF=9.685] Hu, Xiaomeng. et al. PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma. CELL DEATH DIS. 2022 Nov;13(11):1-12 WB ; Human. PubMed:36446769 [IF=7.675] Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443 IF ; Rat. PubMed:36552651 [IF=7.31] Huang Bin. et al. Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization. OXID MED CELL LONGEV. 2022;2022:9735555 WB, IP ; Mouse, Human. PubMed:35993021 [IF=7.129] Zhaoqi He. et al. Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis. ECOTOX ENVIRON SAFE. 2022 Oct;245:114123 WB ; Mouse. PubMed:36183427 [IF=6.023] Lijuan Bao. et al. Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway. Food Chem Toxicol. 2022 May;163:112909 WB ; Mouse. PubMed:35292335 [IF=4.872] Jing Ge. et al. Comparative study on protective effect of different selenium sources against cadmium-induced nephrotoxicity via regulating the transcriptions of selenoproteome. Ecotox Environ Safe. 2021 Jun;215:112135 WB ; PubMed:33780782 [IF=4.872] Xiao Han. et al. ZEB1 directly inhibits GPX4 transcription contributing to ROS accumulation in breast cancer cells. Breast Cancer Res Tr. 2021 Jul;188(2):329-342 WB ; Human. PubMed:34169392 [IF=4.556] Andrea Kreheľová. et al. Characterization of Glutathione Peroxidase 4 in Rat Oocytes, Preimplantation Embryos, and Selected Maternal Tissues during Early Development and Implantation. Int J Mol Sci. 2021 Jan;22(10):5174 WB,IHC ; Rat. PubMed:34068371 [IF=4.014] Zhang Kaixin. et al. MiR-129-3p regulates ferroptosis in the liver of Selenium-deficient broilers by targeting SLC7A11. POULTRY SCIENCE. 2022 Oct;:102271 WB ; Chicken. PubMed:10.1016/j.psj.2022.102271 [IF=3.69] Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873 WB ; Human. PubMed:33485970 [IF=3.405] Jianwei Lei. et al. The protective mechanism of protein kinase R to inhibit neuronal ferroptosis in cerebral injury from subarachnoid hemorrhage. BRAIN BEHAV. 2022 Jul;:e2722 WB ; Rat. PubMed:35894766 [IF=3.231] Yajing Li. et al. Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis. ANIMALS. 2022 Jan;12(21):3026 WB ; Mouse. PubMed:36359150 [IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36. WB ; Mouse. PubMed:29678591 [IF=0] Lan B et al. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine. 2020. IHC-P ; Rat. PubMed:doi:10.1016/j.joim.2020.01.008
研究领域	肿瘤细胞生物免疫学信号转导新陈代谢线粒体
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Pig,Cow)
产品应用	WB=1:500-2000, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	22kDa
细胞定位	细胞浆线粒体
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4: 101-197/197
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site. Function: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Subunit: Monomer. Has a tendency to form higher mass oligomers. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Tissue Specificity: Present primarily in testis. Similarity: Belongs to the glutathione peroxidase family. SWISS: P36969 Gene ID: 2879 Database links: Entrez Gene: 2879 Human Entrez Gene: 625249 Mouse Entrez Gene: 29328 Rat Omim: 138322 Human SwissProt: P36969 Human SwissProt: O70325 Mouse SwissProt: P36970 Rat Unigene: 433951 Human Unigene: 359573 Mouse Unigene: 3647 Rat 谷胱甘肽过氧化物酶（GSH-Px）是机体内广泛存在的一种重要的过氧化物分解酶。硒是GSH-Px酶系的组成成分，它能催化GSH变为GSSG，使有毒的过氧化物还原成无毒的羟基化合物，同时促进H2O2的分解，从而保护细胞膜的结构及功能不受过氧化物的干扰及损害。GSH-Px的活性中心是硒半胱氨酸，其活力大小可以反映机体硒水平。GSH-Px酶系主要包括4种不同的GSH-Px，分别为胞浆GSH-Px、血浆GSH-Px、磷脂氢过氧化物GSH-Px及胃肠道专属性GSH-Px。胞浆GSH-Px由4个相同的分子量大小为22kDa的亚基构成四聚体，每个亚基含有1个分子硒半胱氨酸，广泛存在于机体内各个组织，以肝脏红细胞为最多。它的生理功能主要是催化GSH参与过氧化反应，清除在细胞呼吸代谢过程中产生的过氧化物和羟自由基，从而减轻细胞膜多不饱和脂肪酸的过氧化作用。
产品图片	Sample: Kidney (mouse) Lysate at 40 ug Primary: Anti-GPX4 (bs-3884R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22kD Observed band size: 20 kD Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 19 kD Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 ug Lane 2: Liver (Mouse) Tissue Lysate at 40 ug Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug Lane 4: Heart (Mouse) Tissue Lysate at 40 ug Lane 5: Testis (Rat) Tissue Lysate at 40 ug Lane 6: Liver (Rat) Tissue Lysate at 40 ug Lane 7: Kidney (Rat) Tissue Lysate at 40 ug Lane 8: Heart (Rat) Tissue Lysate at 40 ug Lane 9: U937 (Human) Cell Lysate at 30 ug Lane 10: HepG2 (Human) Cell Lysate at 30 ug Lane 11: MCF-7 (Human) Cell Lysate at 30 ug Lane 12: Huvec (Human) Cell Lysate at 30 ug Lane 13: 293T (Human) Cell Lysate at 30 ug Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 20-22 kD

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记