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Rabbit Anti-Cytochrome P450 2B6  antibody (bs-2352R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2352R
英文名称 Cytochrome P450 2B6
中文名称 细胞色素P450 2B6抗体
别    名 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital inducible; Cytochrome P450 subfamily IIB (phenobarbital inducible); Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6; Cytochrome P450 subfamily IIB polypeptide 6; 1,4-cineole 2-exo-monooxygenase; IIB1; P450; P450 IIB1; P450IIB1.  
Specific References  (1)     |     bs-2352R has been referenced in 1 publications.
[IF=2.8] Man, Shuli, et al. "Combination therapy of cyclophosphamide and Rhizoma Paridis Saponins on anti-hepatocarcinoma mice and effects on cytochrome p450 enzyme expression." Steroids (2013).  WB ;  Rat.  
研究领域 肿瘤  心血管  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  新陈代谢  线粒体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat,Dog,Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 56kDa
细胞定位 细胞浆 细胞膜 线粒体
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cytochrome P450 2B6: 401-491/491 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue Specificity:
Expressed in liver, lung and heart right ventricle.

Post-translational modifications:
Phosphorylation is accompanied by a decrease in enzyme activity (By similarity).

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
P20813

Gene ID:
1555

Database links:

Entrez Gene: 1555 Human

Omim: 123930 Human

SwissProt: P20813 Human

Unigene: 1360 Human



产品图片
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cytochrome P450 2B6 antibody (bs-2352R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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