| 产品编号 | bs-2334R |
| 英文名称 | PXR Rabbit pAb |
| 中文名称 | 孤儿核受体PAR1抗体 |
| 别 名 | BXR; NR1I2; Nuclear receptor subfamily 1 group I member 2; ONR 1; ONR1; Orphan nuclear receptor PAR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR 1; PAR 2; PAR; PAR q; PAR1; PAR2; PARq; Pregnane X receptor; PRR; SAR; Steroid and xenobiotic receptor; SXR. |
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Specific References (6) | bs-2334R has been referenced in 6 publications.
[IF=5.58] Zhou, Xiaoqiao, et al. "Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway." Scientific Reports6 (2016): 27876. other ; Pig.
[IF=5.014] Desirée Bartolini. et al. Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation. Antioxidants-Basel. 2021 Feb;10(2):173 WB ; Human.
[IF=4.757] Xianyu Huang. et al. Transcriptome Analysis of Protection by Dendrobium Nobile Alkaloids (DNLA) against Chronic Alcoholic Liver Injury in Mice. BIOMEDICINES. 2022 Nov;10(11):2800 WB ; Mouse.
[IF=3.407] Mohandas S et al. Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis. Chem Biol Interact. 2019 Nov 4;315:108891. IHC-P ; Mouse.
[IF=2.72] Sehirli, AO, et al. "St. Johns wort may ameliorate 2,4,6-trinitrobenzenesulfonic acid colitis off rats through the induction of pregnane X receptors and/or P-glycoproteins."Journal of Physiology and Pharmacology 66.2 (2015): 203-214. IHC-P ; Rat.
[IF=2.302] Sun B et al.
The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients. Pharmacogenomics. 2019 Feb 25. WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PXR: 51-150/434 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The human nuclear pregnane X receptor (PXR) activates cytochrome P450-3A expression in response to a wide variety of xenobiotics and plays a critical role in mediating dangerous drug-drug interactions. The human PXR is related to the mouse Pxr1, which they had cloned and shown to be activated by dexamethasone, pregnenolone 16-alpha-carbonitrile (PCN), and other compounds known to induce expression of the CYP3A1 gene, the predominant form of CYP3A in rat liver and intestine. Northern blot analysis detected most abundant expression in liver, colon, and small intestine; transcripts of 2.6, 4.3, and 5 kb were present in each of these tissues. Subunit: Heterodimer with RXR. Interacts with NCOA1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in liver, colon and small intestine. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: O75469 Gene ID: 8856 Database links: Entrez Gene: 8856 Human Omim: 603065 Human SwissProt: O75469 Human Unigene: 7303 Human Unigene: 207164 Rat |
| 产品图片 |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti- PXR (bs-2334R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-PXR antibody (bs-2334R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
