产品编号 | bs-3942R |
英文名称 | GNB2 Rabbit pAb |
中文名称 | G蛋白β2抗体/鸟嘌呤核苷酸结合蛋白β亚基2抗体 |
别 名 | G protein beta 2; G protein beta 2 subunit; G protein subunit beta 2; G protein, beta 2 subunit; Gnb2l1; guanine nucleotide binding protein(G protein), beta polypeptide 2; Guanine nucleotide binding protein beta 2 subunit; Guanine nucleotide binding protein G I G S G T beta 2 subunit 2; Guanine nucleotide binding protein G protein beta polypeptide 2; guanine nucleotide binding protein, beta 2 subunit; RACK1; Receptor for activated C kinase; Receptor of activated protein kinase C 1; signal transducing guanine nucleotide binding regulatory protein beta; Transducin beta chain 2; OTTHUMP00000174601; OTTHUMP00000174602; GBB2_HUMAN. |
研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 结合蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 37 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human G protein beta 2: 121-220/340 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
GNB2 belongs to the WD repeat G protein beta family.
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. Interacts with ATXN10. Subcellular Location: Cytoplasm, perinuclear region. Similarity: Belongs to the WD repeat G protein beta family. Contains 7 WD repeats. SWISS: P62879 Gene ID: 2783 Database links: Entrez Gene: 2783 Human Entrez Gene: 14693 Mouse Omim: 139390 Human SwissProt: P62879 Human SwissProt: P62880 Mouse Unigene: 185172 Human Unigene: 30141 Mouse Unigene: 1155 Rat |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNB2) Polyclonal Antibody, Unconjugated (bs-3942R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |