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Rabbit Anti-Lipoprotein lipase  antibody (bs-2336R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2336R
英文名称 Lipoprotein lipase
中文名称 脂蛋白脂酶抗体
别    名 Lipoprotein lipase; LIPD; LIPL_HUMAN; LPL; LPL protein; EC 3.1.1; EC 3.1.1.34; HDLCQ11; LPL; LPL protein; MGC137861.  
Specific References  (1)     |     bs-2336R has been referenced in 1 publications.
[IF=2.752] Xuchun Liu. et al. The Effect of FATP1 on Adipocyte Differentiation in Qinchuan Beef Cattle. Animals-Basel. 2021 Oct;11(10):2789  WB ;  Bovine.  
研究领域 肿瘤  心血管  细胞生物  免疫学  信号转导  干细胞  血管内皮细胞  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Human (predicted: Rabbit,Horse,Cow,Pig,Dog,Mouse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 53kDa
细胞定位 细胞浆 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LPL protein: 301-400/475 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Function:
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

Subunit:
Homodimer. Interacts with APOC2; the interaction activates LPL activity in the presence of lipids. Interacts with GPIHBP1.

Subcellular Location:
Cell membrane, Lipid-anchor, GPI-anchor. Secreted. Note=Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.

Tissue Specificity:
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.

Post-translational modifications:
Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

DISEASE:
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.

Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain.

SWISS:
P06858

Gene ID:
4023

Database links:

Entrez Gene: 280843 Cow

Entrez Gene: 4023 Human

Entrez Gene: 16956 Mouse

Omim: 238600 Human

SwissProt: P11151 Cow

SwissProt: P06858 Human

SwissProt: P11152 Mouse

Unigene: 180878 Human

Unigene: 1514 Mouse



产品图片
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-Lipoprotein lipase (bs-2336R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (bs-2336R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (bs-2336R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (blue line): raji(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Lipoprotein lipase antibody (bs-2336R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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