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Rabbit Anti-PFKFB2/PFK2  antibody (bs-5005R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-5005R
英文名称 PFKFB2/PFK2
中文名称 果糖-2,6-二磷酸酶2/磷酸果糖激酶2抗体
别    名 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2; 6-bisphosphatase; 6-P2ase 2; 6-P2ASE heart-type isozyme; 6PF 2 K/Fru 2 6 P2ASE heart type isozyme; 6PF-2-K/Fru-2; F262_HUMAN; Fructose 2 6 bisphosphatase cardiac isozyme; Fructose-2; PFK 2/FBPase 2; PFK/FBPase 2; PFKFB cardiac; PFKFB2.  
研究领域 肿瘤  心血管  细胞生物  免疫学  信号转导  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Chicken,Dog)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 58kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PFKFB2: 51-150/505 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. It has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFKFB2 regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. There are two named isoforms.

Function:
Synthesis and degradation of fructose 2,6-bisphosphate.

Subunit:
Homodimer (By similarity).

Tissue Specificity:
Heart.

Post-translational modifications:
Phosphorylation by AMPK stimulates activity.

Similarity:
In the C-terminal section; belongs to the phosphoglycerate mutase family.

SWISS:
O60825

Gene ID:
5208

Database links:

Entrez Gene: 5208 Human

Omim: 171835 Human

SwissProt: O60825 Human



产品图片
Sample:
PC-3 Cell Lysate at 40 ug
HepG2 Cell Lysate at 40 ug
Primary: Anti- PFKFB2/PFK2 (bs-5005R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (bs-5005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(bs-5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated(bs-5005R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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