[IF=7.25] Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013).  WB ;  Mouse.  

PubMed:23828893

[IF=4.011] Yuan Qi. et al. Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling. J Diabetes Res. 2022;2022:1755563  WB ;  Human,Mouse.  

PubMed:35132380

[IF=2.955] Shino Oikawa. et al. Non-neuronal cardiac acetylcholine system playing indispensable roles in cardiac homeostasis confers resiliency to the heart. J Physiol Sci. 2021 Dec;71(1):1-20  WB ;  Mouse.  

PubMed:33461483

Sample: Lane 1: Human Hela cell lysates Lane 2: Human U937cell lysates Lane 3: Human HL-60 cell lysates Lane 4: Human HepG2 cell lysates Lane 5: Human SH-SY5Y cell lysates Primary: Anti-Glucose 6 phosphatase alpha (bs-4044R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucose 6 phosphatase alpha) Polyclonal Antibody, Unconjugated (bs-4044R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bs-4044R Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).