| 产品编号 | bs-5053R |
| 英文名称 | CSNK1G2 |
| 中文名称 | 酪蛋白激酶1γ2抗体 |
| 别 名 | Casein kinase 1, gamma 2; KC1G2_HUMAN; Casein kinase I isoform gamma 2; CK1g2; CKI gamma 2; CSNK1G 2; CSNK1G2. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 脂蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse (predicted: Rabbit,Horse,Cow,Pig,Dog,Chicken,Human) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CSNK1G2: 211-320/415 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Casein kinase 1 gamma 2 (CSNK1G2) is a serine/threonine protein kinase which shows preferential utilization of acidic proteins such as caseins as a substrate. It can phosphorylate a large number of proteins. Function: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Subunit: Monomer (By similarity). Interacts with MTA1 (short isoform) in the cytoplasm. Binds to PER1 and triggers its proteasomal degradation. Interacts with SMAD3. Subcellular Location: Cytoplasm. Tissue Specificity: Testis. Post-translational modifications: Autophosphorylated (By similarity). Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain. SWISS: P78368 Gene ID: 1455 Database links: Entrez Gene: 1455 Human Entrez Gene: 103236 Mouse Omim: 602214 Human SwissProt: P78368 Human SwissProt: Q8BVP5 Mouse Unigene: 651905 Human Unigene: 29873 Mouse Unigene: 98151 Rat |
| 产品图片 |
Sample:
Lane 1: Rat Muscle tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-CSNK1G2 (bs-5053R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
Sample:
Uterus (Mouse) Lysate at 40 ug Primary: Anti- CSNK1G2 (bs-5053R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 49 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
