| 产品编号 | bs-5060R |
| 英文名称 | FADS1 Rabbit pAb |
| 中文名称 | 脂肪酸去饱和酶1抗体 |
| 别 名 | delta 5 desaturase; delta 5 fatty acid desaturase; FADS6; FADSD5; D5D antibody Fatty acid desaturase 1; FLJ38956; Linoleoyl CoA desaturase(delta 6 desaturase) like 1; LLCDL1; TU12; FADS1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FADS1: 381-444/444 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FADS1 is a component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids from precursor essential polyunsaturated fatty acids, linoleic acid and alphanlinolenic acid. It catalyzes the desaturation of dihomo gamma linoleic acid and eicosatetraenoic acid to generate arachidonic acid and eicosapentaenoic acid respectively. Function: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein Tissue Specificity: Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina. Similarity: Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain SWISS: O60427 Gene ID: 3992 Database links: Entrez Gene: 3992 Human Entrez Gene: 76267 Mouse Omim: 606148 Human SwissProt: O60427 Human SwissProt: Q920L1 Mouse Unigene: 503546 Human Unigene: 30158 Mouse Unigene: 28161 Rat |
| 产品图片 |
Sample:
Adrenal gland (Mouse) Lysate at 40 ug
Primary: Anti- FADS1 (bs-5060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- FADS1 (bs-5060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-FADS1 (bs-5060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FADS1) Polyclonal Antibody, Unconjugated (bs-5060R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
