| 产品编号 | bs-5085R |
| 英文名称 | MVD |
| 中文名称 | 甲羟戊酸脱羧酶抗体 |
| 别 名 | Diphosphomevalonate decarboxylase; FP17780; MDDase; Mevalonate (diphospho) decarboxylase; Mevalonate (diphospho)decarboxylase; Mevalonate pyrophosphate decarboxylase; MPD; Mvd; MVD1_HUMAN． |
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Specific References (2) | bs-5085R has been referenced in 2 publications.
[IF=2.629] Li Yanchu. et al. Effects of a Chlorogenic Acid-Containing Herbal Medicine (LASNB) on Colon Cancer. Evid-Based Compl Alt. 2021;2021:9923467 IHC ; Mouse.
[IF=1.813] Li Y et al. Study on the Anticancer Effect of an Astragaloside- and Chlorogenic Acid-Containing Herbal Medicine (RLT-03) in Breast Cancer. Evid Based Complement Alternat Med. 2020 Jun 10;2020:1515081. IHC-P ; Human.
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| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 脂蛋白 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Dog,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MVD: 201-300/400 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The enzyme mevalonate pyrophosphate decarboxylase (MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Function: Performs the first committed step in the biosynthesis of isoprenes. Subunit: Homodimer. Tissue Specificity: Expressed in heart, skeletal muscle, lung,liver, brain, pancreas, kidney and placenta. Similarity: Belongs to the diphosphomevalonate decarboxylase family. SWISS: P53602 Gene ID: 4597 Database links: Entrez Gene: 4597 Human Entrez Gene: 192156 Mouse Omim: 603236 Human SwissProt: P53602 Human SwissProt: Q99JF5 Mouse Unigene: 252457 Human Unigene: 28146 Mouse Unigene: 10288 Rat |
| 产品图片 |
Sample:
H9C2(Rat) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-MVD (bs-5085R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD 
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MVD) Polyclonal Antibody, Unconjugated (bs-5085R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
