| 产品编号 | bs-5124R |
| 英文名称 | hHBrk1 Rabbit pAb |
| 中文名称 | 微丝相关蛋白hHBrk1抗体 |
| 别 名 | Brk1-like; C3orf10 chromosome 3 open reading frame 10; C3ORF10 GENE; Chromosome 3 open reading frame 10; hHBrk1; HSPC300; MDS027; Probable protein BRICK1; BRK1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8.7 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human hHBrk1: 31-75/75 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms. In Drosophila, the WAVE/ARC pathway may affect the development of the nervous system. HSPC300 is thought to localize to axons of the central nervous system of Drosophila embryos and thus may also be involved in axonogenesis. In addition, HSPC300 is thought to be necessary for synaptic morphogenesis by motoneurons. In mice, the knockout of the WAVE complex leads to learning and memory defects, and it is therefore hypothesized that HSPC300 may also be involved in cognitive functions. Genetic depletion of HSPC300 results in cytoskeletal abnormalities and prevents cytokinesis of cells, suggesting that decreased levels of HSPC300 may be associated with tumor suppression. Function: Involved in regulation of actin and microtubuleorganization. Part of a WAVE complex that activates the Arp2/3complex. Subunit: Homotrimer when in free form. Directly interacts withWASF2. Component of the WAVE1 complex composed of ABI2, CYFIP1 orCYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, aheterdimer containing NCKAP1 and CYFIP1 interacts with aheterotrimer formed by WAVE1, ABI2 and BRK1. Subcellular Location: Cytoplasm, cytoskeleton Similarity: Belongs to the BRK1 family. SWISS: Q8WUW1 Gene ID: 55845 Database links: Entrez Gene: 55845 Human Entrez Gene: 101314 Mouse Omim: 611183 Human SwissProt: Q8WUW1 Human SwissProt: Q91VR8 Mouse hHBrkl可能参与调控肿瘤细胞迁移能力。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
