| 产品编号 | bs-3793R |
| 英文名称 | CD75 |
| 中文名称 | 唾液酸转移酶1/α-2,6唾液酸转移酶抗体 |
| 别 名 | 6 sialyltransferase; 6-sialyltransferase 1; 6-ST 1; Alpha 2; Alpha 2,6 ST 1; Alpha 2,6 ST; B cell antigen CD75; B-cell antigen CD75; Beta galactoside alpha 2,6 sialyltransferase 1; Beta-galactoside alpha-2; CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; MGC48859; Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase); Sialyltransferase 1; SIAT1; SIAT1_HUMAN; ST6 beta galactosamide alpha 2,6 sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N. |
| 研究领域 | 细胞生物 免疫学 信号转导 转运蛋白 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47kDa |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD75: 301-406/406 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. Function: Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. Similarity: Belongs to the glycosyltransferase 29 family. SWISS: P15907 Gene ID: 6480 Database links: Entrez Gene: 6480 Human Entrez Gene: 20440 Mouse Omim: 109675 Human SwissProt: P15907 Human SwissProt: Q64685 Mouse Unigene: 207459 Human Unigene: 149029 Mouse Unigene: 54567 Rat |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD 
Sample:
MCF-7(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
