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Rabbit IgG (bs-0295P)
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说明书: 下载
10mg/264.00元
50mg/660.00元
100mg/1100.00元
大包装/询价

产品编号 bs-0295P
英文名称 Rabbit IgG
中文名称 兔IgG(亲和层析纯化)
别    名 Rabbit Immunoglobulin G; Normal Rabbit IgG; Native Rabbit IgG  (亲和层析纯化) 兔IgG; 正常兔免疫球蛋白
Specific References  (75)     |     bs-0295P has been referenced in 75 publications.
[IF=17.694] Li, Xinzhi. et al. Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH). NAT COMMUN. 2022 Aug;13(1):1-19  Other ;  Other.  
[IF=17.694] Li, Qing. et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. NAT COMMUN. 2022 Dec;13(1):1-17  
[IF=14.679] Junjie Zhou. et al. TNIP3 is a novel activator of Hippo-YAP signaling protecting against hepatic ischemia/reperfusion injury. 2021 Jun 16  ChIP ;  Mouse.  
[IF=12.11] Shang, Luoran, et al. "Photonic Crystal Microbubbles as Suspension Barcodes." Journal of the American Chemical Society (2016).  other ;  
[IF=11.56] Shen et al. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. (2018) Nucleic.Acids.Res. 46:2290-2307  IF ;  
[IF=10.849] Liu, Xuanyu. et al. Single-cell RNA sequencing identifies an Il1rn+/Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection. Cell Discov. 2022 Feb;8(1):1-21  Other ;  Other.  
[IF=9.66] Zhao, Shuai, et al. "Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology." Proceedings of the National Academy of Sciences (2017): 201704155.  other ;  
[IF=9.58] Xiaoxu Liu. et al. HIF-1–regulated expression of calreticulin promotes breast tumorigenesis and progression through Wnt/β-catenin pathway activation. P Natl Acad Sci Usa. 2021 Nov;118(44):  FC ;  Human.  
[IF=9.518] He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117.  Other ;  
[IF=9.221] Cuixia Guo. et al. Thickness and fluorescence-based dual-encoded suspension array and corresponding decoding system for multiplexed detection. SENSOR ACTUAT B-CHEM. 2023 Apr;:133793  
[IF=8.37] Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).  other ;  
[IF=8.355] Liu X et al. Polydopamine-based molecular imprinted optic microfiber sensor enhanced by template-mediated molecular rearrangement for ultra-sensitive C-reactive protein detection. Chemical Engineering Journal. 2020, May 387: 124074.  interferences ;  
[IF=8.22] Xia, Wenlong, and Jianwei Jiao. "Histone variant H3. 3 orchestrates neural stem cell differentiation in the developing brain." Cell Death & Differentiation (2017).  
[IF=7.78] Zhuang et al. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. (2014) Mol.Cancer. 13:243  Control,IHC ;  
[IF=7.62] Forsberg et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. (2016) Elife. 5  IHC ;  
[IF=6.992] Shuya Luo. et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res. 2021 Mar;:  IHC ;  Human.  
[IF=6.641] Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019.  SERS-based sandwich immunoassay ;  
[IF=6.393] Chen X et al.Dual-digital encoded suspension array based on Raman spectroscopy and laser induced breakdown spectroscopy for multiplexed biodetection.(2019)Sensors and Actuators B: Chemical, 282, 457–468.  other ;  
[IF=6.057] Haohua Yang. et al. Biotin-streptavidin sandwich integrated PDA-ZnO@Au nanocomposite based SPR sensor for hIgG detection. TALANTA. 2022 Aug;246:123496  Other ;  Other.  
[IF=6.042] Zhou Y et al. Cell Cycle-Dependent Uptake and Cytotoxicity of Arsenic-Based Drugs in Single Leukemia Cells. Anal Chem. 2018 Sep 4;90(17):10465-10471.  FCM ;  
[IF=5.923] Paulina Borkowska. et al. Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC. Int J Mol Sci. 2021 Jan;22(13):7086  FC ;  Human.  
[IF=5.9] Braun, Molly, et al. "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." Brain, Behavior, and Immunity (2017).  
[IF=5.743] Yaofei Chen. et al. MoS2-Nanoflower and Nanodiamond Co-Engineered Surface Plasmon Resonance for Biosensing. BIOSENSORS-BASEL. 2023 May;13(5):506  
[IF=5.401] Jiang, Wen-Shuai, et al. "Reduced graphene oxide-based optical sensor for detecting specific protein." Sensors and Actuators B: Chemical (2017).  other ;  
[IF=5.051] Pignatti Emanuele. et al. Cholesterol Deprivation Drives DHEA Biosynthesis in Human Adrenals. ENDOCRINOLOGY. 2022 Jul;163(7):  
[IF=5.047] Du YQ et al. A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI.(2018) Journal of Materials Chemistry B.  CLSM ;  
[IF=5.04] Benesch, Matthew GK, et al. "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." The FASEB Journal 29.9 (2015): 3990-4000.  IHC-P ;  
[IF=5.01] Ma, Hongwei, et al. "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." ACS applied materials & interfaces 2.11 (2010): 3223-3230.  QCM,SPR&Protein Microarray ;  
[IF=5.008] Wang, Huimin, et al. "Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma." Oncotarget (2016).  IP ;  
[IF=5.008] Christensen, Anders, et al. "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." Oncotarget.  FCM ;  
性    状 Lyophilized powder
浓    度 N/A
保存条件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

产品图片
Blank control:NIH/3T3.
Isotype Control:Rabbit IgG(bs-0295P)
Dilution: 1ug/Test;
Primary Antibody (green line): Rabbit Anti- beta-Actin antibody (bs- 0061R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG . The secondary antibody(blue line) used for 40 min at room temperature.Acquisition of 20,000 events was performed.
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