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DAB kit (20×) (C-0010)
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说明书: 下载
0.5ml×2/70.00元
1ml×2/120.00元
大包装/询价

产品编号 C-0010
英文名称 DAB kit (20×)
中文名称 DAB显色试剂盒(20×)
别    名 DAB Substrate; DAB Horseradish Peroxidase Color Development Kit; DAB HRP Color Development Kit;   DAB辣根过氧化物酶显色试剂盒; DAB显色试剂盒; 辣根过氧化物酶(HRP)底物; C02-04001; C24005; DAB试剂盒;
Specific References  (71)     |     C-0010 has been referenced in 71 publications.
[IF=21.95] Shi L et al. A Self‐Pumping Dressing for Draining Excessive Biofluid around Wounds. Adv Mater. 2019 Feb;31(5):e1804187.  IHC-P ;  
[IF=10.75] Yao Xinlei. et al. Tectorigenin targets PKACα to promote GLUT4 expression in skeletal muscle and improve insulin resistance in vitro and in vivo. INT J BIOL SCI. 2023 Mar;19(5):1579-1596  
[IF=8.355] Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109.  IHC ;  
[IF=8.355] Xie S et al. Bacteria-propelled microrockets to promote the tumor accumulation and intracellular drug uptake. Chemical Engineering Journal,2019, 123786.  IHC ;  
[IF=7.634] Ma Yubo. et al. Immature rat testis sustained long-term development using an integrative model. BIOL RES. 2022 Dec;55(1):1-13  Other ;  Other.  
[IF=7.419] Dai Cao. et al. Artesunate promoted anti-tumor immunity and overcame EGFR-TKI resistance in non-small-cell lung cancer by enhancing oncogenic TAZ degradation. BIOMED PHARMACOTHER. 2022 Nov;155:113705  Other ;  Other.  
[IF=6.543] Ma Yu-Bo. et al. Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation. Oxid Med Cell Longev. 2021;2021:1699990  Other ;  Rat.  
[IF=6.491] Song J et al. Deleting MyD88 signaling in myeloid cells promotes development of adenocarcinomas of the colon.Cancer Lett. 2018 Oct 1;433:65-75.  IHC ;  
[IF=6.383] Xie S et al. Bacterial microbots for acid-labile release of hybrid micelles to promote the synergistic antitumor efficacy.Acta Biomater. 2018 Sep 15;78:198-210.  IHC-P ;  
[IF=6.304] Nan Wang. et al. PRMT5/Wnt4 axis promotes lymph-node metastasis and proliferation of laryngeal carcinoma. Cell Death Dis. 2020 Oct;11(10):1-15  
[IF=6.208] Yining Zhang. et al. Activating Wnt/β-Catenin Signaling in Osteocytes Promotes Osteogenic Differentiation of BMSCs through BMP-7. INT J MOL SCI. 2022 Jan;23(24):16045  
[IF=6.208] Baohui Yao. et al. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal. INT J MOL SCI. 2023 Jan;24(5):4611  
[IF=5.793] Yanan Shen. et al. Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice. J Neuroinflamm. 2020 Dec;17(1):1-16  IHC ;  Mouse.  
[IF=5.506] Wang Shufen. et al. FOXD3 confers chemo-sensitivity in ovarian cancer through a miR-335/DAAM1/myosin II axis-dependent mechanism. J OVARIAN RES. 2023 Dec;16(1):1-15  
[IF=5.5] Zhang Y et al. MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway.Cell Physiol Biochem. 2018;48(1):348-360.  IHC-P ;  
[IF=5.5] Yu JL et al. Lentivirus-Mediated Gene Silencing of Lymphocyte Function-Associated Antigen 1 Inhibits Apoptosis of Hippocampal Neurons in Rats with Acute Cerebral Ischemia After Cerebral Lymphatic Blockage.(2018) Cell Physiol Biochem.51(3):1069-1086.  IHC-P ;  
[IF=5.391] Yang C et al. Long noncoding RNA HAGLR acts as a microRNA-143-5p sponge to regulate epithelial-mesenchymal transition and metastatic potential in esophageal cancer by regulating LAMP3. FASEB J. 2019 Jul 15:fj201802543RR.  IHC-P ;  
[IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498.  IHC ;  
[IF=5.047] Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019.  IHC-P ;  
[IF=4.831] Ying Xiao. et al. LINC00265 targets miR-382-5p to regulate SAT1, VAV3 and angiogenesis in osteosarcoma.. Aging-Us. 2020 Oct 31; 12(20): 20212–20225  IHC ;  Human.  
[IF=4.694] Qi SS et al. Protective Effects of Chromium Picolinate Against Diabetic-Induced Renal Dysfunction and Renal Fibrosis in Streptozotocin-Induced Diabetic Rats. Biomolecules. 2020 Mar 4;10(3).  staining ;  rat.  
[IF=4.658] Wang AH et al. Down‐regulation of long non‐coding RNA HOTAIR inhibits invasion and migration of oesophageal cancer cells via up‐regulation of microRNA‐204. J Cell Mol Med. 2019 Aug 7.  IHC ;  
[IF=4.545] Dai Caoet al. Luteolin suppresses epithelial-mesenchymal transition and migration of triple-negative breast cancer cells by inhibiting YAP/TAZ activity. Biomed Pharmacother . 2020 Sep;129:110462.  other ;  
[IF=4.522] Ma S et al. Histone deacetylases inhibitor MS-275 suppresses human esophageal squamous cell carcinomacell growth and progression via the PI3K/Akt/mTOR pathway. J Cell Physiol. 2019 May 23.  IHC-P ;  
[IF=4.486] Wang N et al. Dysregulated YY1/PRMT5 axis promotes the progression and metastasis of laryngeal cancer by targeting Hippo pathway  IHC ;  
[IF=4.432] Pengfei Cheng. et al. A3 adenosine receptor agonist IB-MECA reverses chronic cerebral ischemia-induced inhibitory avoidance memory deficit. Eur J Pharmacol. 2022 Apr;921:174874  Other ;  Other.  
[IF=4.255] Chen LD et al. T-cell death-associated gene 8 accelerates atherosclerosis by promoting vascular smooth muscle cell proliferation and migration. Atherosclerosis. 2020 Jan 22;297:64-73.  IHC ;  
[IF=3.998] Hongbin Guo. et al. Metabolites profiling and pharmacokinetics of troxipide and its pharmacodynamics in rats with gastric ulcer. Sci Rep-Uk. 2020 Aug;10(1):1-21  other ;  
[IF=3.973] Luo X et al. Encapsulation of Escherichia coli strain Nissle 1917 in a chitosan―alginate matrix by combining layer-by-layer assembly with CaCl2 cross-linking for an effective treatment of inflammatory bowel diseases. Colloids Surf B Biointerfaces. 2020 Jan 21;189:110818.  IHC-P ;  
[IF=3.923] Liu L et al. CP-31398 inhibits the progression of cervical cancer through reversing the epithelial mesenchymal transition via the downregulation of PAX2s. J Cell Physiol. 2019 Mar;234(3):2929-2942.  IHC ;  
保存条件 Store at –20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 DAB (3,3′-Diaminobenzidine; 3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl) is Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually. The kit contains three components: the DAB Chromogen, Substrate Buffer and Staining Assistant. All components are in liquid form. This new 3-Solution DAB product has improved stability, ease of formulation, and greater sensitivity than previous DAB products.

DAB,即3,3’-二氨基联苯胺(3,3’-diaminobenzidine)是辣根过氧化物酶(Peroxidase)的常用生色底物,在过氧化氢的存在下失去电子而呈现出颜色变化,形成浅棕色不溶性产物。用于检测过氧化物酶的活性,它灵敏度高,特异性好,是HRP结合物最常用的底物,常在免疫组化,原位杂交,Western blot等膜显色或检测细胞或组织内源性的过氧化物酶中应用广泛。
用法:
1. 临用前配制 10 mL 1×DAB 显色工作液:将 0.5 mL 20× DAB 显色液 A 加入 9 mL 1×PBS 中并涡旋混匀。向 DAB / PBS 混合物中加入0.5 mL 20×DAB显色液B涡旋混合均匀。 可以根据需要按比例缩放体积。 注意:不要使用含有叠氮化钠的缓冲液,叠氮化钠是一种HRP抑制剂。
2. 在样品的最后一次洗涤完毕后,去除洗涤液,加入足够 的 DAB 显色工作液,确保能充分覆盖样品。室温避光孵育 3-30 分钟或更长时间,直至显色达到预期深浅。免疫印迹实验,应保证样品在一个足够多的染色液中且能被自由晃动。
3. 去除 DAB 染色工作液,用 PBS 冲洗样品几次以中止显色反应。对于用显微镜观察的细胞或组织样品,可复染样品(可选)并安装在水性封固介质中。或者,可以将样品脱水并安装在有机封固剂中。免疫印迹实验,可将样品用水冲洗,风干,然后在室温下储存。

储存与运输:
-20℃避光密闭保存,一年有效。避免反复冻融。
2~8℃运输,在途时间5天对试剂无影响。

注意事项
1. DAB对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。
2. 显色工作液应现用现配,新鲜配制的工作液应为无色或浅棕色,如颜色过深,请勿使用。  
3. 显色时间严格控制,根据情况调整,以免显色过度。固相膜显色数小时后即会褪色,不能永久存在。
4. 请将沾有DAB显色液A的容器等放在含有3% KMnO4,2% NaHCO3 的溶液浸泡3h,以减少污染。 
5. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
6. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
产品图片
大鼠脑 阳性细胞为棕黄色,阴性细胞为蓝色。
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